FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761397

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761397
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences626203
Sequences flagged as poor quality0
Sequence length20-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT7150.1141802259011854No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC1400.041.9941741
CTTATAC1500.040.6318741
TCGTATG1600.038.8366840
GTATCAA19900.037.94351
CTCGTAT1600.036.61053539
GGTATCA8500.035.8516581
TGCCGTC1750.033.86602445
TATCAAC22650.033.635662
AGGGGGG3200.033.4220570
TCAACGC23100.033.1296464
ATCAACG23150.033.0554543
CAACGCA23550.032.4965935
GTGGTAT3850.031.6612051
AGACGTA1950.031.29027225
AACGCAG24550.031.1704166
TCTCGTA1950.029.98823738
TATACAC2200.029.242643
ACGCAGA26250.029.1517587
TGGTATC4150.028.5565412
AGAGTAC26900.028.31921211
Produced by FastQC (version 0.11.5)