FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005761399

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761399
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1541672
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT51220.3322366884784831No Hit
CTGTTAGCCTGTTGGCAATAGTAAGTTGCAAAATCTTCAGGCTGCAGGCCGCTGATGGTGAGAGTGAAAT40850.26497205631288623No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG40410.26211801213228236No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC37250.24162078574430879No Hit
GGCTAACAGTTTCCCGTGGGCGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT36470.23656134378778362No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC32990.2139884489048254No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGCC31820.20639928597003776No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC31120.20185876113725876No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC30760.199523634080401No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT30730.19932904015899622No Hit
GTCTCTGGTGGCTCCATCAGCAGTAGTAGTTACTACTGGGGCTGGATCCG30090.19517770316902688No Hit
GGATATTAGCAGTTGGTTAGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTGATCTCTGCTGCATC28930.18765340487470744No Hit
GAAATATACAGCCGTGTCTGCGGCGGTCACAGAACTCAGCTCCAGGGAGA28910.18752367559377092No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT28810.18687502918908822No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGCCCACGGGAAA27750.1799993772994515No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC26570.17234534972419555No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCCAGATGCGACAT26570.17234534972419555No Hit
GTTCAGAGGCAGTGGATCTCAGACAGATTTCACTCTCACCATCAGCGGCCTGCAGCCTGAAGATTTTGCAACTTA26140.1695561701840599No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG25790.16728590776767044No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT25770.1671561784867339No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG25760.1670913138462656No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG25610.16611834423924154No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC25600.16605347959877328No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT25100.16281024757535972No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT24930.16170754868739914No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT24690.16015079731616064No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT24550.15924269234960484No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG23960.15541567856197686No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG23920.1551562200001038No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG23900.15502649071916724No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA23430.15197785261715852No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG23170.1502913719649835No Hit
GATTTCACTCTCACCATCAGCGGCCTGCAGCCTGAAGATTTTGCAACTTACTATTGCCAACAGGCTAACAGTTTC22430.14549138857033142No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT21530.13965357092818706No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC21500.13945897700678225No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCCAGATGCGACATCC21470.13926438308537742No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA21300.13816168419741684No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC20930.13576169250009082No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT20730.1344643996907254No Hit
GTATATTTCTGTGCGAGACTTAGGATAGAAGTAGTCCAGAACTTCTACTACTACTACGGTTTGGACGTCTGGGGC20620.1337508886455744No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG20110.13044279198169262No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG19700.1277833417224935No Hit
CTGGGGTCCCGTCAAGGTTCAGAGGCAGTGGATCTCAGACAGATTTCACT19550.12681037211546944No Hit
CACCTGCACTGTCTCTGGTGGCTCCATCAGCAGTAGTAGTTACTACTGGG19410.12590226714891364No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT18740.12155633623753952No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC18690.12123201303519814No Hit
CACCATCAGCGGCCTGCAGCCTGAAGATTTTGCAACTTACTATTGCCAACAGGCTAACAGTTTCCCGTGGGCGTT18550.12032390806864235No Hit
GTCGGAGACACAGTCACCATCACTTGTCGGGCGAGTCAGGATATTAGCAGTTGGTTAGCCTGGTATCAGCAGAAA18480.11986985558536446No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA17620.11429149650509315No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17520.11364285010041046No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA17130.11111312912214791No Hit
GTCTCCATCTTCCGTGTCTGCATCTGTCGGAGACACAGTCACCATCACTT17030.1104644827174652No Hit
CGGGAAACTGTTAGCCTGTTGGCAATAGTAAGTTGCAAAATCTTCAGGCT16990.11020502415559211No Hit
GCTAATATCCTGACTCGCCCGACAAGTGATGGTGACTGTGTCTCCGACAG16940.10988070095325077No Hit
GATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCTAACC16930.10981583631278248No Hit
GAGTGAAATCTGTCTGAGATCCACTGCCTCTGAACCTTGACGGGACCCCA16820.1091023252676315No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT16810.10903746062716324No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT16790.1089077313462267No Hit
GTGCAAACTGGATGCAGCAGAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCTAACCAA16640.10793476173920265No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG16410.1064428750084324No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCTACAGCAAGCTCACCGTGGACAAGAG16370.10618341644655932No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA16230.10527531148000353No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG16170.1048861236371939No Hit
GTATCAACGCAGAGTACGGGATGCTTTCTGAGAGTCATGGATCTCATGTGCAAGAAAATGAAGCACCTGTGGTT15930.1033293722659554No Hit
ACTACAACCCGTCCCTCAAGAGTCGAGTCACCATCTCCGTGGACACGTCC15920.10326450762548714No Hit
CCTTGGTCCCTTGGCCGAACGCCCACGGGAAACTGTTAGCCTGTTGGCAATAGTAAGTTGCAAAATCTTCAGG15850.10281045514220924No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT15800.10248613193986789No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA15710.10190235017565345No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA15550.10086451592816112No Hit
CTCCTGATCTCTGCTGCATCCAGTTTGCACACTGGGGTCCCGTCAAGGTT15440.10015100488301014No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)