FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761400

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761400
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1541672
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT50400.3269177879600849No Hit
CTGTTAGCCTGTTGGCAATAGTAAGTTGCAAAATCTTCAGGCTGCAGGCCGCTGATGGTGAGAGTGAAAT39560.25660451769247933No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG34960.22676678307707474No Hit
GGCTAACAGTTTCCCGTGGGCGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT34410.22319922785131985No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC32980.21392358426435715No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT32880.21327493785967444No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC31100.20172903185632224No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC30730.19932904015899622No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC29910.194010139640598No Hit
GGATATTAGCAGTTGGTTAGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTGATCTCTGCTGCATC29860.19368581643825664No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT29830.19349122251685183No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG28900.18745881095330263No Hit
GTCTCTGGTGGCTCCATCAGCAGTAGTAGTTACTACTGGGGCTGGATCCG28340.18382639108707946No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT27690.17961018945664187No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG27510.178442625928213No Hit
GAAATATACAGCCGTGTCTGCGGCGGTCACAGAACTCAGCTCCAGGGAGA27090.17571831102854563No Hit
GTTCAGAGGCAGTGGATCTCAGACAGATTTCACTCTCACCATCAGCGGCCTGCAGCCTGAAGATTTTGCAACTTA26300.17059400443155223No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCCAGATGCGACAT25010.1622264658111453No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC24970.16196700724927224No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGCC24710.16028052659709718No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT24340.15788053489977116No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGCCCACGGGAAA24310.15768594097836636No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT24110.15638864816900092No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC23630.15327514542652393No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT23350.15145893549341236No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT23250.15081028908872962No Hit
GATTTCACTCTCACCATCAGCGGCCTGCAGCCTGAAGATTTTGCAACTTACTATTGCCAACAGGCTAACAGTTTC23070.14964272556030075No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT22750.1475670570653161No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG22480.1458157117726728No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG22430.14549138857033142No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA22220.14412923112049775No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG22030.1428968029516006No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC21710.14082113445661593No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG21660.1404968112542746No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG21560.13984816484959187No Hit
GTATATTTCTGTGCGAGACTTAGGATAGAAGTAGTCCAGAACTTCTACTACTACTACGGTTTGGACGTCTGGGGC20720.13439953505025712No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC20680.13414007648838405No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT20630.13381575328604267No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG20610.13368602400510615No Hit
CTGGGGTCCCGTCAAGGTTCAGAGGCAGTGGATCTCAGACAGATTTCACT20540.13323197152182825No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA20540.13323197152182825No Hit
GTCGGAGACACAGTCACCATCACTTGTCGGGCGAGTCAGGATATTAGCAGTTGGTTAGCCTGGTATCAGCAGAAA20100.13037792734122433No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCCAGATGCGACATCC20090.13031306270075607No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG19550.12681037211546944No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA19040.12350227545158764No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT19040.12350227545158764No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC18600.1206482312709837No Hit
GTCTCCATCTTCCGTGTCTGCATCTGTCGGAGACACAGTCACCATCACTT18540.12025904342817409No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT17960.11649689428101437No Hit
CACCATCAGCGGCCTGCAGCCTGAAGATTTTGCAACTTACTATTGCCAACAGGCTAACAGTTTCCCGTGGGCGTT17800.11545906003352203No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG17770.11526446611211723No Hit
CACCTGCACTGTCTCTGGTGGCTCCATCAGCAGTAGTAGTTACTACTGGG17710.1148752782693076No Hit
GAGTGAAATCTGTCTGAGATCCACTGCCTCTGAACCTTGACGGGACCCCA17510.1135779854599422No Hit
CTCCTGATCTCTGCTGCATCCAGTTTGCACACTGGGGTCCCGTCAAGGTT17490.11344825617900565No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA17370.11266988049338642No Hit
GTATCAACGCAGAGTACGGGATGCTTTCTGAGAGTCATGGATCTCATGTGCAAGAAAATGAAGCACCTGTGGTT16970.11007529487465557No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT16120.10456180043485255No Hit
CGGGAAACTGTTAGCCTGTTGGCAATAGTAAGTTGCAAAATCTTCAGGCT16070.1042374772325112No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT15930.1033293722659554No Hit
GCTAATATCCTGACTCGCCCGACAAGTGATGGTGACTGTGTCTCCGACAG15700.10183748553518517No Hit
GTGCAAACTGGATGCAGCAGAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCTAACCAA15690.1017726208947169No Hit
GATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCTAACC15680.10170775625424862No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15510.10060505736628803No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA15440.10015100488301014No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTA15420.10002127560207359No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCATT200.0076690751.2134713
GTATCAA37450.041.8009221
GTGGTAT3750.038.2816121
TATCAAC41450.037.8139082
TACATCG750.001965270836.5132370
TCAACGC43400.036.0291984
ATCAACG43450.035.9842383
CAACGCA44150.035.4160045
AACGCAG44500.034.9061626
AGAGTAC46650.033.0789411
CGCAGAG47050.033.0132568
CAGAGTA47600.032.48836510
GCAGAGT47950.032.180039
CGATGTA553.4411173E-431.0717323
TGTTAGA13750.030.37236870
ACGCAGA52300.029.6349967
AGTACGG43900.028.46489113
GAGTACG44000.028.24500712
GAGTACT10800.027.5035312
AGTACTT10950.026.8149713
Produced by FastQC (version 0.11.5)