FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761402

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761402
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2056761
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG30670.14911795779869416No Hit
GTAATACACAGCCGTGTCCTCGGTTCTCAGGCTGTTCATTTGCAGAAACA30210.14688143153239488No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT8300.034.7944871
GTATCAA59300.034.3802641
ATCAACG58150.034.0884933
TATCAAC58850.034.0423852
TCAACGC59550.033.2862784
CAACGCA59900.033.034435
AACGCAG61750.032.154446
GGTATCA26150.031.6850621
ACGCAGA63250.031.5005057
AGAGTAC66850.029.70209311
CGCAGAG67200.029.699318
CAGAGTA67350.029.53115710
GCAGAGT69900.028.4538389
AGTACGG66650.025.35923213
GAGTACG68900.024.68068312
TTCGGGG11100.023.91358470
TACGGGT7250.023.6932315
TGGTATC12800.022.2826082
GTAATAC11650.022.1332171
TACGGGG34950.022.11707115
Produced by FastQC (version 0.11.5)