FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761405

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761405
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1862956
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA48550.038.560361
GGTATCA22550.037.48811
TATCAAC56350.033.5228962
ATCAACG57750.032.587333
TCAACGC58050.032.2992974
CAACGCA59400.031.5652245
AACGCAG61400.030.6476656
CTCGTAT2600.030.57307439
ACGCAGA65050.028.9280057
AGAGTAC66100.028.46848311
CTTATAC4050.027.8870091
CAGAGTA67850.027.88549810
CGCAGAG68250.027.5716728
GCAGAGT70250.026.9328259
TCGTATG3350.026.85320940
GTGGTAT8050.026.7847541
AGTACGG52700.025.1897713
GAGTACG53700.024.72068812
CGTATGC3800.024.61647241
GGTTACG1250.001382370724.49191770
Produced by FastQC (version 0.11.5)