FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761408

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761408
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences544466
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG36520.6707489540210041No Hit
GAATACACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTA10400.19101284561386753No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC9940.18256420051940803No Hit
CTATAATCCTTATTCGTGGACGTTCGGCCAGGGGACCAAGGTGGAGGTCAAACGAACTGTGGCTGCACCAT9930.18238053432170234No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC9150.1680545709006623No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT8910.1636465821557269No Hit
GGATTATAGTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGTATTCTGTC8520.15648360044520687No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC7490.13756598208152576No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT6940.12746434120771546No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC6510.11956669470637284No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG6490.11919936231096157No Hit
GAGTGTATTCTGTCCCAGATCCACTGCCACTGAATCTTGATGGGACTCCA6140.1127710453912641No Hit
CTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA6080.11166904820503025No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAACTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT5920.10873038904173997No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG5800.10652639466927227No Hit
GAGTATCAGTAGCTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCC5720.10505706508762715No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG5640.103587735505982No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG5610.10303673691286508No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT5540.10175107352892558No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT5510.10120007493580868No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGGTT750.001570653838.20817670
GTGGTAT3600.037.3280451
GTATCAA20500.037.2525441
CTAAACG450.0021157135.9652367
TATCAAC22400.033.9120982
GTATAAT502.5136568E-432.7758451
ATCAACG24150.030.903863
TCAACGC23950.030.8970954
CAACGCA24200.030.442615
AACGCAG24450.029.9974176
AGAGTAC25600.029.28938711
GTCGGGT450.005894155729.17702520
CGCAGAG25550.028.8287988
ACGCAGA25550.028.700677
GCAGAGT26150.028.54299
GTGTATA758.7285196E-526.2206761
TATACCC500.00998986626.1869543
GGTATCA13650.026.1726531
TACGGGT10600.025.65702615
GAGTACG25100.025.56801812
Produced by FastQC (version 0.11.5)