FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761409

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761409
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences401707
Sequences flagged as poor quality0
Sequence length20-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGT4440.11052832039272405No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGACAT200.00812274750.46740721
CGCGTAG851.8189894E-1243.73109428
ACGCGTA851.8189894E-1243.69284427
GCGTAGT955.456968E-1239.16700729
GTATCAA19350.038.7646181
TCGTATG1009.094947E-1238.18871740
GACGCGT1001.2732926E-1137.1064626
GCCGTCT1102.1827873E-1135.21012546
ACCGATT400.002515674334.7170140
CTCGTAT1003.619789E-1034.6502339
TATCAAC22350.034.6148072
TCTCGTA1056.311893E-1032.9031738
TCAACGC23600.032.6348044
ATCAACG23650.032.565813
ACGAGAC1156.366463E-1132.18211722
CAACGCA23950.032.1578875
TATGCCG1206.548362E-1132.09610443
CGTATGC1206.91216E-1131.93059541
GGTATCA7800.031.4803711
AGACGCG1201.0186341E-1030.90276125
Produced by FastQC (version 0.11.5)