FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761411

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761411
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31725
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGT7532.3735224586288415No Hit
ACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTCT2180.6871552403467297Illumina Paired End PCR Primer 2 (95% over 24bp)
ATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTT2060.6493301812450749Illumina Paired End PCR Primer 2 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATGTCGTATGCCGT980.30890464933018125No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG760.23955870764381404No Hit
GTATCAACGCAGAGTACGGGTGCCTGCGCAGAGAAATGG640.20173364854215917No Hit
CCATTTCTCTGCGCAGGCACCCGTACTCTGCGTTGATAC500.15760441292356187No Hit
ATACACATCTCCGAGCCCACGAGACACTGAGCGATGTCGTATGCCGTCTT330.10401891252955082No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT320.10086682427107958No Hit
ACACATCTCCGAGCCCACGAGACACTGAGCGATGTCGTATGCCGTCTTCT320.10086682427107958RNA PCR Primer, Index 1 (95% over 21bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGAAGA100.009773401129.8279370
TTTGTCA100.009773401129.8279370
ACGGGTC150.00331227263.17061216
TCGCGAT200.004388956458.80441362
AGGGGGG1550.058.63196670
TACTTTA200.00588005554.6270540
CTTATAC1100.045.8698041
CATTTCT501.3508907E-744.1496854
CCATTTC501.3508907E-744.1496853
GCCATTT501.3508907E-744.1496852
TTATACA1250.040.3654252
TATACAC1300.038.8129123
TTTCTCT506.9059115E-637.8425876
TCTGCGC506.9059115E-637.84258710
GTATCAA2950.037.4149861
AAGGGGG1700.037.25166369
TCTCTGC605.649381E-736.79148
GTCGTAT400.002046825836.0893539
TACGGGT1150.035.64881515
TGGTATC451.6622852E-435.0394332
Produced by FastQC (version 0.11.5)