FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761412

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761412
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31725
Sequences flagged as poor quality0
Sequence length20-76
%GC59

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19566.16548463356974No Hit
CCATTTCTCTGCGCAGGCACCCGTACTCTGCGTTGATAC650.2048857368006304No Hit
GTATCAACGCAGAGTACGGGTGCCTGCGCAGAGAAATGG490.15445232466509062No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACATCT150.00307579164.362784
TACACAT150.00307579164.362782
ACACATC150.00307579164.362783
GTGATCG350.005897937354.48063770
CGTCGGG350.005897937354.48063770
AAGTCCA200.006039262354.26329842
AACGGCG250.006905153452.4274169
GACAAAA350.001031569441.49059343
GGTGCCT1700.039.87928419
GTATCAA4300.038.9170271
TACGGGG1100.038.03254715
ACGGGGT703.780042E-836.8368316
GGGTGCC1850.036.58793318
GTGCCTG1950.036.42210420
GAAATGG853.2960088E-935.88567433
CGGGTGC1900.035.62509517
TGCCTGC1850.034.90078721
AAATGGC905.484253E-934.0617734
TACGGGT2000.033.79045515
TATCAAC5000.033.4686432
Produced by FastQC (version 0.11.5)