FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761413

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761413
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2135345
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAGCAGTATGGTGGCTCATTTTACACTTTTGGCCAGGGGA90900.42569233543057444No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT76410.3578344482975819No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA75570.35390065773914753No Hit
CTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA55570.2602389777764249No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC54850.2568671572977669No Hit
GTAATATACGGCCGTGTCTGCGGCGGTCACAGAGGTCAGCTTCAGGGAGA53580.250919640620134No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG52170.24431649218276202No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGTT52010.24356719874306024No Hit
GTGTATTACTGTCAGCAGTATGGTGGCTCATTTTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA50200.23509081670643386No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC49210.2304545635482791No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC45700.21401693871482128No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA44920.21036413319627506No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT44420.208022591197207No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATT44300.2074606211174307No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG41690.19523777188229535No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG41000.19200644392358143No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCGGGGCCAGTCAGAGTCTTACCGGTCCGAGTCTTAGCGG40840.19125715048387965No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC40510.1897117327644947No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC39980.18722969824548258No Hit
CTCCTGCGGGGCCAGTCAGAGTCTTACCGGTCCGAGTCTTAGCGGCGCCTACTTAGCCTGGTACCAGCAGAAAC39460.18479449456645178No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT39020.18273393760727188No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT38370.1796899330084834No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG38240.1790811320887257No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCT38090.1783786694890053No Hit
GTCTCTGGTGGCTCCATCAGTAGTTACTACTGGAGCTGGATCCGGCAGCC37800.1770205751295458No Hit
GAGTCTTACCGGTCCGAGTCTTAGCGGCGCCTACTTAGCCTGGTACCAGCAGAAACCTGGCCTGGCGCCCAGGCT36960.17308678457111146No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT34750.1627371689352306No Hit
TACTTAGCCTGGTACCAGCAGAAACCTGGCCTGGCGCCCAGGCTCCTCATCTATGATGCATCCAACAGGGCCA34050.1594590101365353No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT33700.15781993073718767No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT33650.15758577653728087No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG32880.15397980185871604No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG32080.15023333466020714No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG32000.14985868794035626No Hit
GTATATTACTGTGCGAGAACCGGTTTTTGGACTGGTTATTTTGACTTCTGGGGCCAGGGAACCCAGGTCACCGT31890.14934354870056127No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCA31870.14924988702059855No Hit
GACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATTTTGCAGTGTA31640.14817277770102721No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG31480.14742348426132545No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA31280.14648686746169823No Hit
GTATGGTGGCTCATTTTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT31140.14583123570195916No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC30980.14508194226225737No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTTCTCCTGGTGGCAGCTCCCAGATGGGTC30720.143864340422742No Hit
GCTCACTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT30390.14231892270335705No Hit
CACTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC30040.1406798433040094No Hit
GTCAGAGTCTTACCGGTCCGAGTCTTAGCGGCGCCTACTTAGCCTGGTAC29940.1402115349041958No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT28630.13407669486663748No Hit
GGCTAAGTAGGCGCCGCTAAGACTCGGACCGGTAAGACTCTGACTGGCCCCGCAGGAGAGGGTGGCTCTTTCCC28560.13374887898676796No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC27610.1292999491885386No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT27500.12878480994874364No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG27260.12766086978919097No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA27070.1267710838295451No Hit
CTGCTACTCTGGCTCCCAGATACCACCGGAGACATTGTGTTGACGCAGTC26380.12353975587083119No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA26350.12339926335088709No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG25990.12171335311155808No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT25920.12138553723168857No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACCGGAGACATTGTGTTGACGCAGTCTC25830.1209640596718563No Hit
TCTATGATGCATCCAACAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACT25270.11834153263290006No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC25170.11787322423308647No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG24460.1145482345944098No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT24280.11370527947474529No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT24260.11361161779478257No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC24100.1128623243550808No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA24020.1124876776352299No Hit
TCATAGATGAGGAGCCTGGGCGCCAGGCCAGGTTTCTGCTGGTACCAGGCTAAGTAGGCGCCGCTAAGACTCGGA23910.11197253839543492No Hit
GGGCAGTGGTGGGTGCTTTATTTCCATGCTGGGTGCCTGGGAAGTATGTA23390.10953733471640414No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA23340.10930318051649733No Hit
GTGTTGACGCAGTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGC23000.10771093195713105No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG22940.10742994691724289No Hit
GTGTAAAATGAGCCACCATACTGCTGACAGTAATACACTGCAAAATCTTC22450.10513523575815617No Hit
CATCTATGATGCATCCAACAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA22320.10452643483839848No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT22320.10452643483839848No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC22280.10433911147847304No Hit
GTATCAACGCAGAGTACGGGGAGGAACTGCTCACTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTT22240.1041517881185476No Hit
ACCCTCTCCTGCGGGGCCAGTCAGAGTCTTACCGGTCCGAGTCTTAGCGG21660.10143559939962864No Hit
GCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACCGGAGACATTGTGTTGACGCAGTCTCCAGCCACC21630.10129510687968454No Hit
GTCTGGGATGCCAGTGGCCCTGTTGGATGCATCATAGATGAGGAGCCTGGGCGCCAGGCCAGGTTTCTGCTGGTA21600.10115461435974046No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC2050.046.9125981
GGTATCA16950.044.174551
CGTATGC2050.043.8389441
TCGTATG2150.041.7841640
ATCTCGT2150.040.11091637
GTATCAA39600.038.7701641
TATCTCG2300.037.4711936
GCTTGTA2850.034.94931
TATCAAC44900.034.34272
AACGCGA400.002657813534.3370712
AGCCTAT2650.033.76431332
TTATACA2900.033.158472
ATGCCGT2650.032.66499744
GCCTATC2650.032.4786433
TCAACGC48100.032.127094
ATCAACG48200.032.0604363
TACACAT3150.031.6148685
TATACAC2950.031.4300963
CAACGCA49050.031.364835
AACGCAG49800.031.0274736
Produced by FastQC (version 0.11.5)