FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761414

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761414
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2135345
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAGCAGTATGGTGGCTCATTTTACACTTTTGGCCAGGGGA82510.3864012606862123No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT76560.35853691089730233No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA69810.3269260939098834No Hit
CTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA54400.2547597694986056No Hit
GTAATATACGGCCGTGTCTGCGGCGGTCACAGAGGTCAGCTTCAGGGAGA49380.23125068782796224No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGTT49040.22965843926859592No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC48570.22745738978947197No Hit
GTGTATTACTGTCAGCAGTATGGTGGCTCATTTTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA46450.21752925171342335No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG46420.21738875919347928No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT46000.2154218639142621No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC45570.21340813779506357No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC44250.20722646691752386No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATT43990.20600886507800847No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA42780.20034233344026373No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCGGGGCCAGTCAGAGTCTTACCGGTCCGAGTCTTAGCGG40400.18919659352469975No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG39710.18596526556598583No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT39020.18273393760727188No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC39010.18268710676729052No Hit
CTCCTGCGGGGCCAGTCAGAGTCTTACCGGTCCGAGTCTTAGCGGCGCCTACTTAGCCTGGTACCAGCAGAAAC37870.17734839100941535No Hit
GAGTCTTACCGGTCCGAGTCTTAGCGGCGCCTACTTAGCCTGGTACCAGCAGAAACCTGGCCTGGCGCCCAGGCT37610.17613078916989994No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT37530.17575614245004906No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT36420.17055791921211794No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC35190.1647977258944105No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG35160.16465723337446642No Hit
GTCTCTGGTGGCTCCATCAGTAGTTACTACTGGAGCTGGATCCGGCAGCC35050.16414209413467146No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCT34260.1604424577761439No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG34060.15950584097651668No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT33890.15870971669683354No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG32050.15009284214026306No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCA31660.14826643938098993No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT31480.14742348426132545No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG31300.14658052914166095No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA30610.143349201182947No Hit
GTATATTACTGTGCGAGAACCGGTTTTTGGACTGGTTATTTTGACTTCTGGGGCCAGGGAACCCAGGTCACCGT30540.1430213853030775No Hit
GCTCACTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT30220.14152279842367393No Hit
TACTTAGCCTGGTACCAGCAGAAACCTGGCCTGGCGCCCAGGCTCCTCATCTATGATGCATCCAACAGGGCCA30020.14058618162404668No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT29990.14044568910410263No Hit
GACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATTTTGCAGTGTA29800.13955590314445673No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA29670.13894710222469905No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC29590.13857245550484817No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTTCTCCTGGTGGCAGCTCCCAGATGGGTC28650.1341703565466002No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG28460.13328057058695433No Hit
GTATGGTGGCTCATTTTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT28110.1316414911876067No Hit
GTCAGAGTCTTACCGGTCCGAGTCTTAGCGGCGCCTACTTAGCCTGGTAC27920.1307517052279608No Hit
CACTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC27880.13056438186803537No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG27690.1296745959083895No Hit
GGCTAAGTAGGCGCCGCTAAGACTCGGACCGGTAAGACTCTGACTGGCCCCGCAGGAGAGGGTGGCTCTTTCCC27520.12887847162870636No Hit
CTGCTACTCTGGCTCCCAGATACCACCGGAGACATTGTGTTGACGCAGTC27180.1272862230693401No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG26520.12419538763057024No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA26440.12382074091071935No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT26340.12335243251090572No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG26220.1227904624311294No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC25630.12002744287222908No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT25590.11984011951230364No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT25310.1185288559928255No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACCGGAGACATTGTGTTGACGCAGTCTC25160.11782639339310509No Hit
GTGTTGACGCAGTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGC25030.1172175924733474No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA24000.11239401595526718No Hit
GTATCAACGCAGAGTACGGGGAGGAACTGCTCACTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTT23440.10977148891631096No Hit
GGGCAGTGGTGGGTGCTTTATTTCCATGCTGGGTGCCTGGGAAGTATGTA23430.10972465807632958No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC23180.10855388707679556No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT22590.10579086751789524No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG22470.1052288974381189No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG22290.10438594231845438No Hit
TCTATGATGCATCCAACAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACT22040.10321517131892037No Hit
GTCTGGGATGCCAGTGGCCCTGTTGGATGCATCATAGATGAGGAGCCTGGGCGCCAGGCCAGGTTTCTGCTGGTA21820.10218489283933042No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA21670.10148243023961No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG21660.10143559939962864No Hit
GCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACCGGAGACATTGTGTTGACGCAGTCTCCAGCCACC21570.10101412183979638No Hit
GTGTAAAATGAGCCACCATACTGCTGACAGTAATACACTGCAAAATCTTC21400.10021799756011324No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16400.042.9028971
GTATCAA42600.040.9289551
TTAACGA350.001318902439.6044345
TATCAAC48250.035.9669842
ATCAACG50800.034.212233
TCAACGC51500.033.679864
CAACGCA51800.033.4154935
AACGCAG52400.033.1644746
AGAGTAC55200.031.42012411
CGCAGAG55700.031.198158
GCAGAGT56400.030.8717149
CGTATCC450.004702799530.56032631
AGGTACG450.004759263730.485852
AACTGTG38750.029.24098470
ACGCAGA62050.028.0619837
CGTTTAA500.00790114327.48105429
CAGAGTA63900.027.14099510
TAATCGT2550.027.05715238
GCTTGTC3750.026.542591
GAGTACG58550.026.5202712
Produced by FastQC (version 0.11.5)