FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761416

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761416
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1022879
Sequences flagged as poor quality0
Sequence length20-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG18300.17890679151688518No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18600.038.869581
GTATCAA39350.033.7992321
TATCAAC44750.029.5292032
ATCAACG44450.029.48713
CAACGCA44450.029.4076355
TCAACGC44900.029.1129044
AACGCAG45000.029.049636
ACGCAGA46300.028.233987
GTGGTAT7450.027.4651451
CGCAGAG48100.027.1053098
AGAGTAC48250.026.95444711
CGTTAAG950.00960592226.39847670
CAGAGTA49450.026.22764810
GCAGAGT50200.026.1070429
GAGTACG38450.025.50119412
AGTACGG38750.025.21713613
TGGTATC8000.023.8402632
GTACGGG42200.022.91350214
TACGGGG14650.022.31428115
TACGGGA16700.020.5946415
Produced by FastQC (version 0.11.5)