FastQCFastQC Report
Wed 8 May 2024
EGAF00005761417

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761417
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences504160
Total Bases36.9 Mbp
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTACACAGCCGTGTCCGCGGCGGTCACAGAGGTCAAGTTCAGGGAGA8380.16621707394477944No Hit
GTATAACAGTGCCCCTCGAACGTTCGGCCAAGGGACCAAGGTGGAAATCA7470.14816724849254204No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC7280.14439860361789908No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA6880.1364646144081244No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT6850.1358695652173913No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC6400.12694382735639478No Hit
CTGTTATACTTTTGACAGTAATAAGTTGCAACATCTTCAGGCTGCAGGCT5900.11702634084417646No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC5820.11543954300222152No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGT5530.10968740082513487No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC5510.10929070136464615No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG5140.10195176134560457No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)