FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761418

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761418
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences504160
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13770.27312757854649317No Hit
GTAGTACACAGCCGTGTCCGCGGCGGTCACAGAGGTCAAGTTCAGGGAGA7250.14380355442716597No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT7080.14043160901301174No Hit
CTGTTATACTTTTGACAGTAATAAGTTGCAACATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGAGAAATCTGTC5780.11464614408124404No Hit
GTATAACAGTGCCCCTCGAACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT5750.11405109489051093No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC5070.100563313233894No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT2400.046.2086071
GTATCAA14250.034.6675031
CGGGATA400.002959923633.58624317
TATGGAT2657.2759576E-1231.43477470
ATCAACG15950.030.7252253
TCAACGC16100.030.236484
CAACGCA16250.029.9603445
GGAACGA450.00525047429.87519522
AACGCAG16650.029.4422346
TATCAAC16550.029.4172532
CTGATAT500.005773376729.360
TTCGACT500.00716998628.0289948
GTCCGAT1800.028.02748125
CAGAGTA17650.027.9615810
AGAGTAC17550.027.93237511
CGCAGAG17950.027.3072198
GTGTCTA1950.026.99006865
GCAGAGT18350.026.8949269
AGTCGCG500.00879536626.87966721
ACGCAGA18650.026.2848937
Produced by FastQC (version 0.11.5)