FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005761420

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761420
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences882269
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT57480.6515019795549883No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG35260.3996513534987628No Hit
GTGCAGCCACAGTTCGCTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTC34940.3960243417823816No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC34430.3902437918593989No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC33820.3833298007750471No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT33340.37788928320047516No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC31250.35420036292785984No Hit
CTATAAAGCTGTTGACAGGAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATTGTGAGAGTGAATTCTGTC30020.3402590366430193No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGGGCATTAGCAGTTATTTAGCCTGGTATCAGCAAAAA29190.33085147500365536No Hit
GTCTCCATCCTTCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT28940.3280178721002325No Hit
GCTTTATAGTTTTCCTCGGACTTTTGGCCAGGGGACCAAGCTGGAGATCAAGCGAACTGTGGCTGCACCAT28910.32767783975182174No Hit
GATCTATGCTGCATCCACTTTACAAAATGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCA28800.3264310544743157No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC28380.32167060159656524No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT28040.3178169016479101No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT27370.31022284586673676No Hit
CTCCTGATCTATGCTGCATCCACTTTACAAAATGGGGTCCCATCAAGGTT26260.2976416489755392No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC26150.29639486369803314No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT26100.29582814311734856No Hit
GTCCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG26060.2953747666528009No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG25320.2869873020586692No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC25290.28664726971025845No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACAT24400.276559643374073No Hit
ATGCTGCATCCACTTTACAAAATGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCA24260.2749728257481562No Hit
GAATTCACTCTCACAATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTATTCCTGTCAACAGCTTTATAGTTTT24020.27225256696087025No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG23850.2703257169865427No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT23560.2670387376185721No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG22620.2563843907017021No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG22600.25615770246942826No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT22230.2519639701723624No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA22040.249810431965761No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG21800.24709017317847506No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA21220.24051621444253396No Hit
GATCAGGAGTTTAGGGGCTTTCCCTGGTTTTTGCTGATACCAGGCTAAAT20950.23745592330683724No Hit
GCATAGATCAGGAGTTTAGGGGCTTTCCCTGGTTTTTGCTGATACCAGGC20200.22895511459656862No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT19980.2264615440415565No Hit
GGGCATTAGCAGTTATTTAGCCTGGTATCAGCAAAAACCAGGGAAAGCCCCTAAACTCCTGATCTATGCTGCATC19080.21626057358923412No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA18710.21206684129216827No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC18650.21138677659534674No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACATCCAGTTGACCCAGTCTCCATCCTTC18560.21036667955011457No Hit
ACTTATTCCTGTCAACAGCTTTATAGTTTTCCTCGGACTTTTGGCCAGGGGACCAAGCTGGAGATCAAGCGAA18160.20583291490463793No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG18110.20526619432395338No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG18090.2050395060916795No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGCTTGATCTCCAGCTTGGTCC18080.2049261619755426No Hit
ACCTTGATGGGACCCCATTTTGTAAAGTGGATGCAGCATAGATCAGGAGTTTAGGGGCTTTCCCTGGTTTTTG18010.20413275316258422No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT17870.2025459355366674No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA17670.20027905321392908No Hit
GTTTAGGGGCTTTCCCTGGTTTTTGCTGATACCAGGCTAAATAACTGCTA17650.20005236498165527No Hit
CATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACAATCAGCAGCCTGCAGCCTGAAGATTTTG17540.19880557970414917No Hit
GGCCAAAAGTCCGAGGAAAACTATAAAGCTGTTGACAGGAATAAGTTGCA17080.19359175036185108No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG16720.18951136218092215No Hit
GTATCAACGCAGAGTACGGGAGTCCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCT16460.18656441516136235No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA16190.18350412402566565No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16080.18225733874815958No Hit
GTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCATTTTGTAAAGT15550.17625010059290308No Hit
GGAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATTGTGAGAGTGAATTCTGTCCCAGATCCACTGCCG15550.17625010059290308No Hit
GCCACAGTTCGCTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTCCGAGG15400.17454993885084932No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA14690.16650250660512836No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC14650.1660491301405807No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC14570.1651423772114854No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT14280.16185539784351485No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCC14150.16038192433373494No Hit
ATGCAGCATAGATCAGGAGTTTAGGGGCTTTCCCTGGTTTTTGCTGATACCAGGCTAAATAACTGCTAATGCC14060.1593618272885027No Hit
CCCCTAAACTCCTGATCTATGCTGCATCCACTTTACAAAATGGGGTCCCA13790.15630153615280598No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA13190.1495008891845911No Hit
CTCACAATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTATTCCTGTCA13150.14904751272004343No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA13140.1489341686039065No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC13040.14780072744253736No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT13020.14757403921026355No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC13020.14757403921026355No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA12960.14689397451344205No Hit
GTCAGGGCATTAGCAGTTATTTAGCCTGGTATCAGCAAAAACCAGGGAAAGCCCCTAAACTCCTGATCTATGCTG12910.14632725393275747No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC12890.14610056570048363No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT12800.1450804686552514No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA12780.14485378042297758No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT12700.14394702749388225No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC12650.14338030691319767No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAGCGAACTGTGGCTGCACCA12600.14281358633251312No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC12530.1420201775195547No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC12510.14179348928728086No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC12450.14111342459045936No Hit
CTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACATCCAGTTGACCCAGTC12450.14111342459045936No Hit
ATCTCCAGCTTGGTCCCCTGGCCAAAAGTCCGAGGAAAACTATAAAGCTG12150.13771310110635193No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC12150.13771310110635193No Hit
GTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACAATCAGCAGCC12130.1374864128740781No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA12110.13725972464180425No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT12080.1369196922933935No Hit
ATCTGGGACAGAATTCACTCTCACAATCAGCAGCCTGCAGCCTGAAGATT12070.1368063481772566No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT12070.1368063481772566No Hit
GCTTGGTCCCCTGGCCAAAAGTCCGAGGAAAACTATAAAGCTGTTGACAGGAATAAGTTGCAAAATCTTCAGG12010.13612628348043512No Hit
CACAATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTATTCCTGTCAAC11840.13419943350610755No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11820.13397274527383374No Hit
CCATCCTTCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCG11730.13295264822860148No Hit
GAGTACGGGAGTCCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCT11660.13215923941564306No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC11650.13204589529950617No Hit
GCTCTGGCTCCCAGGTGCCAGATGTGACATCCAGTTGACCCAGTCTCCATCCTTCCTGTCTGCATCTGTAGGAGA11290.1279655071185772No Hit
GTGCCAGATGTGACATCCAGTTGACCCAGTCTCCATCCTTCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCA11230.1272854424217557No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGG11220.12717209830561882No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG11130.1261520012603866No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT11090.1256986247958389No Hit
GATTGTGAGAGTGAATTCTGTCCCAGATCCACTGCCGCTGAACCTTGATG11050.12524524833129125No Hit
GAGTCACCATCACTTGCCGGGCCAGTCAGGGCATTAGCAGTTATTTAGCCTGGTATCAGCAAAAACCAGGGAAAG11020.1249052159828805No Hit
GAGTGAATTCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCA10900.12354508658923753No Hit
CTCCAGCTTGGTCCCCTGGCCAAAAGTCCGAGGAAAACTATAAAGCTGTT10860.12309171012468986No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC10820.1226383336601422No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAG10680.1210515160342254No Hit
CACCATCACTTGCCGGGCCAGTCAGGGCATTAGCAGTTATTTAGCCTGGTATCAGCAAAAACCAGGGAAAGCCC10650.12071148368581464No Hit
CTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGGGCATTAGC10610.12025810722126698No Hit
GCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTCGCTTGATCTCCAGCTTGGTCCCCTGGC10370.11753784843398102No Hit
GTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA10340.11719781608557028No Hit
GCTGATTGTGAGAGTGAATTCTGTCCCAGATCCACTGCCGCTGAACCTTG10210.11572434257579038No Hit
GCAGTGGATCTGGGACAGAATTCACTCTCACAATCAGCAGCCTGCAGCCT10120.11470424553055815No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT10000.11334411613691515No Hit
GTCTCCTACAGATGCAGACAGGAAGGATGGAGACTGGGTCAACTGGATGTCACATCTGGCACCTGGGAGCCAGAG9890.1120973308594091No Hit
AGTCAGGGCATTAGCAGTTATTTAGCCTGGTATCAGCAAAAACCAGGGAAAGCCCCTAAACTCCTGATCTATG9870.11187064262713525No Hit
CAGTTGACCCAGTCTCCATCCTTCCTGTCTGCATCTGTAGGAGACAGAGT9840.11153061027872452No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC9830.1114172661625876No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACG9750.11051051323349229No Hit
GGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTG9710.11005713676894462No Hit
GCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACATCCAGTTGACCCAGTCTCCATC9510.10779025444620632No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT9420.10677015740097409No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG9410.10665681328483716No Hit
GTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACT9360.10609009270415258No Hit
GTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGG9150.10370986626527738No Hit
GGACCAAGCTGGAGATCAAGCGAACTGTGGCTGCACCATCTGTCTTCATC9140.10359652214914045No Hit
GGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCT9110.10325648980072971No Hit
GGCTCCCAGGTGCCAGATGTGACATCCAGTTGACCCAGTCTCCATCCTTC9030.10234973687163439No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGCTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTCCGAGGAAAA9020.10223639275549748No Hit
GTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGC9000.10200970452322364No Hit
GCCCCTAAACTCCTGATCTATGCTGCATCCACTTTACAAAATGGGGTCCCATCAAGGTTCAGCGGCAGTGGAT9000.10200970452322364No Hit
TATTTAGCCTGGTATCAGCAAAAACCAGGGAAAGCCCCTAAACTCCTGAT8900.10087626336185448No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)