FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761422

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761422
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences615991
Sequences flagged as poor quality0
Sequence length20-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7280.11818354488945454No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATTGT556.375784E-445.89590570
GGTATCA9200.041.0151
GTGGTAT3050.040.4893231
TATACGA350.001343035439.45414736
TTATACG350.001347904839.42511435
GTATCAA21550.038.2033841
TGGTATC3400.034.267242
CGAATTA501.9368337E-434.26445815
ATCAACG25450.031.9057623
GTCGTGC652.435044E-531.6210258
ACTTTCG1003.1390766E-431.55343270
TATCAAC26100.031.5101032
TCAACGC26050.031.4313834
CAACGCA26000.031.360065
AACGCAG26600.030.1375226
ACGCAGA27250.029.4186447
AGAGTAC28100.028.16071711
CGCAGAG28550.027.9568238
GCAGAGT29500.026.8220989
CAGAGTA29800.026.66485610
Produced by FastQC (version 0.11.5)