FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761424

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761424
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences498944
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12550.25153123396613647No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT6260.12546498204207285No Hit
GAGTTACAGTACCCCTCGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT5460.10943111852231913No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA20200.039.9340931
AACGTTG350.001483586738.661827
TATCAAC22050.036.535912
TCAACGC23350.034.3396034
CCCGTCG400.002699292534.2236135
ATCAACG23600.033.9826553
CAACGCA23700.033.832485
AACGCAG23700.033.6829726
GTGGTAT2600.032.5900421
ACGCAGA24750.032.2572337
AGAGTAC24550.032.2476611
ACGGGCT1600.031.73067516
GCAGAGT25500.030.9074049
CGCAGAG25750.030.6103978
GGTATCA9750.030.5911871
TACGGGT4750.029.205715
AGTACGG23250.028.52400613
CGGGTGC2750.028.32465417
GAGTACG23800.027.86483812
TATTCTA950.00740400127.84020870
Produced by FastQC (version 0.11.5)