FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761425

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761425
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1798219
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18500.040.9442561
GTATCAA43700.040.47071
ATCAACG50500.034.611073
TCGTATG1300.034.5891740
TATCAAC50900.034.5410732
TCAACGC51000.034.27274
CAACGCA52500.033.358765
AACGCAG54750.031.9878526
ACGCAGA57450.030.424857
AGAGTAC59450.029.68707811
CGCAGAG59350.029.5646958
CAGAGTA61450.028.7774310
CTCGTAT1601.8189894E-1228.07454539
GCAGAGT63700.027.7071579
AGTACGG50600.026.5474913
GAGTACG51650.026.075612
CGCTTAT809.964679E-525.71954524
GTGGTAT6450.025.5066831
CGTATGC1851.8189894E-1124.3352741
GTACGGG56450.023.6762414
Produced by FastQC (version 0.11.5)