FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761430

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761430
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences905010
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13470.1488381343852554No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA23300.037.868281
GGTATCA10350.036.4880521
ATCAACG26150.033.9605033
CAACGCA26800.033.007075
TCAACGC26800.033.007074
TATCAAC26900.032.900732
AACGCAG28450.031.3338056
TGGTATC4400.031.1849772
ACGCAGA29150.030.346127
GTGGTAT5100.030.2928891
AGAGTAC29400.029.74149911
CGCAGAG30100.029.5006318
CAGAGTA30550.029.29053710
TTAACGG1103.6714482E-828.2193635
CGACGGA1107.191913E-626.69146269
GCAGAGT33700.026.4509669
AGTACGG29250.025.67510813
CGCGGTA1101.0950025E-625.23818643
GAGTACG30000.025.03323212
TACGGGA10600.023.61756115
Produced by FastQC (version 0.11.5)