FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005761433

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761433
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences958984
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA20990.21887747866492038No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT20830.21720904624060464No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC17040.1776880531896257No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG15560.16225505326470513No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC14620.1524530127718502No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAGGATT13760.14348518849115313No Hit
GCCTAGAGCCTGAGGATTTTGCAGTTTATTACTGTCACCAGCGTAGCAACTGGCCTCCCACTTTCGGCGGAGGGA12710.13253610070658114No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC12590.13128477638834432No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT12570.13107622233530486No Hit
GTCTCCAGCCCTCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTCTTAGCAG11950.1246110466910814No Hit
CTCCTGCAGGGCCAGTCAGAGTCTTAGCAGCTACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCT11870.12377683047892353No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG11700.12200412102808807No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG11660.12158701292200912No Hit
GTAATAAATGGCCGTGTCCGCGGCAGTCATAGAGGCCAGTTTCAGGGAGA11530.1202314115772526No Hit
GTCTTTGGTGGCTCCATCAGCAGTGGTGGTTACTTCTGGACCTGGGTCCG10950.11418334403910804No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG10940.11407906701258833No Hit
GACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAGGATTTTGCAGTTTATTACTGTCACCAGCGTAGCAACTGG10830.11293201972087125No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10600.1105336481109174No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT10480.10928232379268057No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA10370.10813527650096351No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC10330.10771816839488459No Hit
ATGTATACCCGCCCCGACTTCTCTCGCACAGTAATAAATGGCCGTGTCCG10310.10750961434184511No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT10210.10646684407664779No Hit
GTTTATTACTGTCACCAGCGTAGCAACTGGCCTCCCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAA10190.10625829002360833No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG10190.10625829002360833No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG10130.10563262786448993No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC9780.10198293193629926No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT9780.10198293193629926No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCT9700.10114871572414139No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)