FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005761434

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761434
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences958984
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT21200.2210672962218348No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA19080.19896056659965128No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG14890.15526849248788302No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC14410.1502631952149358No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13270.1383756141916862No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC12830.13378742502481794No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC12500.13034628314966673No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAGGATT12460.12992917504358778No Hit
GCCTAGAGCCTGAGGATTTTGCAGTTTATTACTGTCACCAGCGTAGCAACTGGCCTCCCACTTTCGGCGGAGGGA12210.12732224938059444No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT11900.12408966155848272No Hit
GTCCCAGACCCACTGCCGCTGAACCTGGCTGGGATGCCAGTGGCCCTGTT11740.12242122913416699No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT11090.11564322241038433No Hit
GTTTATTACTGTCACCAGCGTAGCAACTGGCCTCCCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAA10620.11074220216395685No Hit
GTCTTTGGTGGCTCCATCAGCAGTGGTGGTTACTTCTGGACCTGGGTCCG10600.1105336481109174No Hit
CTCCTGCAGGGCCAGTCAGAGTCTTAGCAGCTACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCT10330.10771816839488459No Hit
GTAATAAATGGCCGTGTCCGCGGCAGTCATAGAGGCCAGTTTCAGGGAGA10320.10761389136836486No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC10250.10688395218272671No Hit
GTCTCCAGCCCTCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTCTTAGCAG10250.10688395218272671No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG9990.10417274949321366No Hit
GACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAGGATTTTGCAGTTTATTACTGTCACCAGCGTAGCAACTGG9880.1030257022014966No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC9850.10271287112193737No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9770.10187865490977953No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT9650.10062733059154272No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA9650.10062733059154272No Hit
ATGTATACCCGCCCCGACTTCTCTCGCACAGTAATAAATGGCCGTGTCCG9630.10041877653850324No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG9610.1002102224854638No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)