FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761436

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761436
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2095744
Sequences flagged as poor quality0
Sequence length20-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG24010.11456551945275759No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA58050.040.446031
GGTATCA25750.039.0582161
TATCAAC69100.033.852142
TCAACGC69250.033.5183684
ATCAACG69200.033.493043
CAACGCA69650.033.275065
AACGCAG72350.032.3175966
GTGGTAT9100.030.931041
ACGCAGA76050.030.7001937
CGCAGAG77450.030.0117368
AGAGTAC78150.029.70188311
CAGAGTA81100.028.70329910
GCAGAGT83300.028.0686979
AGTACGG69550.025.9799213
GAGTACG71500.025.27137612
TGGTATC11250.025.0000652
GTACGGG79050.022.42399614
TACGGGG31800.021.35080315
TACGGGT14800.021.31582815
TACGGGA24950.021.1653915
Produced by FastQC (version 0.11.5)