FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761437

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761437
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences635339
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG306.1839976E-446.1875640
GTATCAA16000.037.425891
GGTATCA7500.037.4116331
CTAACGG450.003862597331.81091565
TATCAAC19150.031.8057252
GTGGTAT2700.031.6832941
ATCAACG19350.031.2976843
CGTCCGA450.0043818331.0017345
CAACGCA19550.030.97755
TCAACGC19650.030.8174294
CTCGTAT450.004563693430.74515239
CTATATA450.00461366230.67679637
AACGCAG20500.029.537316
ACGCAGA21400.028.4549457
AGAGTAC22450.027.27647411
CGCAGAG22700.026.976078
AGTACGG17100.026.80563213
GAGTACG17300.026.49573912
CAGAGTA23200.026.3946910
GCAGAGT23900.025.7647619
Produced by FastQC (version 0.11.5)