FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761438

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761438
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences635339
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6730.1059277015892303No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCAAAT200.005212604556.46038468
GGTATCA7950.035.3512041
GTATCAA15850.033.7328341
CAACGCA17050.030.112235
TCAACGC17100.030.0265484
ATCAACG17250.029.7677883
TATCAAC17800.029.2372422
AACGCAG17950.028.6024276
AGAGTAC19500.026.50651411
ACGCAGA19400.026.4646157
CGCAGAG20150.025.819318
CAGAGTA20300.025.45991910
AGTACGG16800.024.65393813
GTGGTAT3350.024.5540961
ACGGGTA700.001395572924.4501916
GCAGAGT21200.024.217629
TACGGGG7400.023.59112715
GAGTACG17600.023.53330412
ACGGGGG3350.023.50137716
TAACGGC1052.276337E-523.0030436
Produced by FastQC (version 0.11.5)