FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761445

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761445
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences829877
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT25660.3092024480736302No Hit
GTAATAAACGGCCGTGTCCGCGGCAGTCACAGAAGTCAGGTTCAGGGAAA21030.2534110476612799No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTCCTCTGGCTCCCAGATATCA20180.2431685659441098No Hit
GTATGATAACTGGCCTTGGACGTTCGGCCAAGGGACCACGGTGGAAATCAAACGAACTGTGGCTGCACCAT18060.21762261154363838No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG17130.20641613154720517No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC16600.20002964294708733No Hit
GTCTGAAGACTCTGCAGTTTATTACTGTCAGCAGTATGATAACTGGCCTT15340.18484667004869398No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT14330.1726761917729977No Hit
GTCTGGGACAGAGTTCACGCTCACCATCAGCAGCCTGCAGTCTGAAGACT14040.16918169801066904No Hit
GTTATCATACTGCTGACAGTAATAAACTGCAGAGTCTTCAGACTGCAGGCTGCTGATGGTGAGCGTGAACT13870.16713320166723503No Hit
GTCTCTATCTCCGGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTTACAGTGTTAGCAGCCACCTAGCCTGGTA13860.16701270188232714No Hit
CATCTATGGTGCGTCCAGCATGGCCTCTGGTATCCCAGCCAGGTTCAGTG13540.16315670876527485No Hit
GTCTTCAGACTGCAGGCTGCTGATGGTGAGCGTGAACTCTGTCCCAGACC13490.16255420984073543No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT13240.15954171521803834No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT13120.15809571779914375No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACACCTGTGGTTCTTCCTCCTGCTGGTGGCAGCTCCCAGATGGGTC13070.15749321887460432No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATACCAGAGGCCATGCT13050.15725221930478855No Hit
GTGTTAGCAGCCACCTAGCCTGGTACCAACAAAAACCTGGCCAGGCTCCC12410.14954023307068398No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC12360.14893773414614456No Hit
CCATAGATGAGGAGCCGGGGAGCCTGGCCAGGTTTTTGTTGGTACCAGGC11930.14375624339510554No Hit
GTTTATTACTGTCAGCAGTATGATAACTGGCCTTGGACGTTCGGCCAAGGGACCACGGTGGAAATCAAACGAA11340.1366467560855404No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT11320.13640575651572462No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT11170.13459825974210637No Hit
CTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCACG11160.1344777599571985No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG11120.13399576081756695No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG11110.13387526103265907No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC10830.13050126705523832No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC10810.13026026748542255No Hit
TCTATGGTGCGTCCAGCATGGCCTCTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCACG10710.1290552696363437No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG10610.12785027178726485No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC10540.12700677329290966No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT10460.12604277501364658No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG10380.12507877673438353No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10150.1223072816815022No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCGCAGCTTCTCT10090.1215842829720549No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCGTGGTCCCTTGGCCGAACGTC9990.12037928512297605No Hit
GTTACAGTGTTAGCAGCCACCTAGCCTGGTACCAACAAAAACCTGGCCAGGCTCCCCGGCTCCTCATCTATGGTG9950.11989728598334454No Hit
CTATTACACTGGGAATACCTACTACAACCCGTCCCTCAAGAGTCGAGTTGCCATTTCAATAGACACGTCTAAGAA9870.11893328770408146No Hit
GTTTATTACTGTGCGAGAGCCCTCCGAATGGCGTCCTATTTTGACTACTGGGGCCAGGGAATCCTGGTCACCGT9850.11869228813426569No Hit
CTCCTGCAGGGCCAGTTACAGTGTTAGCAGCCACCTAGCCTGGTACCAACAAAAACCTGGCCAGGCTCCCCGGCT9760.11760779007009473No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG9610.11580029329647647No Hit
GTAATAAACTGCAGAGTCTTCAGACTGCAGGCTGCTGATGGTGAGCGTGAACTCTGTCCCAGACCCACTGCCA9550.11507729458702916No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG9310.11218529974923995No Hit
GAGTTCACGCTCACCATCAGCAGCCTGCAGTCTGAAGACTCTGCAGTTTATTACTGTCAGCAGTATGATAACTGG8880.10700380899820094No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA8810.10616031050384575No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG8660.10435281373022749No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA8430.10158131867734617No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT8340.1004968206131752No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT1800.047.7234041
GGTATCA5350.043.6736071
GTATCAA14350.042.382381
TCGTATG856.548362E-1140.6474440
TATTGAA6950.039.387370
TATCAAC17150.035.458532
CTCGTAT1102.7284841E-1134.529439
AGACGGA905.3769327E-934.3732825
GTATATT400.002647876534.3608551
ATCAACG18100.033.597453
TCAACGC18350.033.1397174
CGTATGC1056.2755134E-1032.9310141
CTTATAC1154.9112714E-1132.8669051
CAACGCA18900.032.53895
TGTACTA751.7235961E-632.0662655
AACGCAG19300.031.8625976
TCGATGC553.3378735E-431.23149313
CGAGACG1001.5035766E-830.93222423
AGTCTAA450.004717015730.53929915
GTACGCT450.004717015730.5392994
Produced by FastQC (version 0.11.5)