FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005761446

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761446
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences829877
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT23690.28546399044677706No Hit
GTAATAAACGGCCGTGTCCGCGGCAGTCACAGAAGTCAGGTTCAGGGAAA20970.2526880489518326No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTCCTCTGGCTCCCAGATATCA19670.2370230769138077No Hit
GTATGATAACTGGCCTTGGACGTTCGGCCAAGGGACCACGGTGGAAATCAAACGAACTGTGGCTGCACCAT17810.2146101169209413No Hit
GTCTGAAGACTCTGCAGTTTATTACTGTCAGCAGTATGATAACTGGCCTT15340.18484667004869398No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT15070.1815931758561811No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT15000.1807496773618259No Hit
GTCTGGGACAGAGTTCACGCTCACCATCAGCAGCCTGCAGTCTGAAGACT14450.1741221891918923No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC14110.17002519650502423No Hit
GTCTCTATCTCCGGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTTACAGTGTTAGCAGCCACCTAGCCTGGTA13970.16833819951631385No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG13960.16821769973140596No Hit
GTCTTCAGACTGCAGGCTGCTGATGGTGAGCGTGAACTCTGTCCCAGACC13770.16592820381815618No Hit
GTTATCATACTGCTGACAGTAATAAACTGCAGAGTCTTCAGACTGCAGGCTGCTGATGGTGAGCGTGAACT13580.1636387079049064No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATACCAGAGGCCATGCT12290.14809423565178936No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACACCTGTGGTTCTTCCTCCTGCTGGTGGCAGCTCCCAGATGGGTC12120.14604573930835535No Hit
CATCTATGGTGCGTCCAGCATGGCCTCTGGTATCCCAGCCAGGTTCAGTG11850.1427922451158425No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT11410.13749025457989558No Hit
GTTTATTACTGTCAGCAGTATGATAACTGGCCTTGGACGTTCGGCCAAGGGACCACGGTGGAAATCAAACGAA11180.13471875952701426No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC11100.13375476124775118No Hit
GTGTTAGCAGCCACCTAGCCTGGTACCAACAAAAACCTGGCCAGGCTCCC11030.132911262753396No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG10960.1320677642590408No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT10840.1306217668401462No Hit
CCATAGATGAGGAGCCGGGGAGCCTGGCCAGGTTTTTGTTGGTACCAGGC10780.1298987681306989No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT10720.12917576942125159No Hit
CTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCACG10700.12893476985143582No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC10690.12881427006652793No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG10680.12869377028162005No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC10630.12809127135708062No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC10600.12772977200235697No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG10030.12086128426260759No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10030.12086128426260759No Hit
TCTATGGTGCGTCCAGCATGGCCTCTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCACG9950.11989728598334454No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT9900.11929478705880511No Hit
CTCCTGCAGGGCCAGTTACAGTGTTAGCAGCCACCTAGCCTGGTACCAACAAAAACCTGGCCAGGCTCCCCGGCT9760.11760779007009473No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9680.11664379179083166No Hit
GTTACAGTGTTAGCAGCCACCTAGCCTGGTACCAACAAAAACCTGGCCAGGCTCCCCGGCTCCTCATCTATGGTG9650.116282292436108No Hit
GAGTTCACGCTCACCATCAGCAGCCTGCAGTCTGAAGACTCTGCAGTTTATTACTGTCAGCAGTATGATAACTGG9470.11411329630776608No Hit
CTATTACACTGGGAATACCTACTACAACCCGTCCCTCAAGAGTCGAGTTGCCATTTCAATAGACACGTCTAAGAA9340.1125467991039636No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG9280.1118238003945163No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCGTGGTCCCTTGGCCGAACGTC9200.11085980211525323No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA8970.10808830706237191No Hit
GTTTATTACTGTGCGAGAGCCCTCCGAATGGCGTCCTATTTTGACTACTGGGGCCAGGGAATCCTGGTCACCGT8940.10772680770764825No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG8870.10688330921329306No Hit
GTAATAAACTGCAGAGTCTTCAGACTGCAGGCTGCTGATGGTGAGCGTGAACTCTGTCCCAGACCCACTGCCA8820.10628081028875364No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCGCAGCTTCTCT8730.10519631222458267No Hit
GTGTAATAGATGTACCCAATCCACTCCAGGCTCTTCCCTGGGTGCTGGCGGATCCAGCTCCAGTAGTAATCACCA8640.10411181416041172No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA8600.10362981502078018No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT8330.10037632082826732No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)