FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761447

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761447
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences660604
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATACAGCCGTGTCTTCCCCTCTCAGGCTGTTCATTTGCAGATACA16520.2500741745432967No Hit
CTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGT14350.2172254482261688No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC13700.2073859679929277No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT13070.19784924099763246No Hit
GTATATTACTGTGCGAGAAAGGGGTACAACCATGGTTACGATCGGGGCAATTGGGCAGAGTGGGACTACTTTGA12140.18377121543314906No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG11980.181349189529582No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC11490.17393173519990796No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA11390.17241796901017856No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT10970.1660601510133151No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC10770.1630326186338563No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC10630.16091334596823512No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT10150.153647268257534No Hit
GTTCCAATACCCCGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGA9940.1504683592591023No Hit
GTATTGGAACTCTGTTGACAGTAATAAATTGCAAAATCTTCAGGTTGCAGATTGCTGATGGTGAGAGTGAAAT9480.14350503478634705No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG9270.14032612578791528No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG9050.1369958401705106No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG8950.13548207398078124No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC8670.13124352864953892No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT8590.1300325156977554No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT8530.12912425598391775No Hit
GTGAGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGACACAGATTTCACT8440.12776186641316128No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG8310.12579397036651307No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG8140.12322056784397309No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC7960.12049578870246018No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG7880.11928477575067664No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA7830.11852789265581196No Hit
GTGCAAACTGGATGCAGCAAAGATCAGGAGTTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATTTAAATAG7730.11701412646608256No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT7680.11625724337121786No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC7640.11565173689532608No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCTACAGCAAGCTCACCGTGGACAAGAG7610.11519760703840728No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT7510.11368384084867787No Hit
GATCAGGAGTTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATTTAAAT7500.11353246422970494No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTGGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTAGCAG7390.1118673214210026No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG7220.10929391889846261No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT7040.1065691397569497No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG7040.1065691397569497No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT7010.1061150099000309No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT6910.10460124371030148No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT6820.10323885413954502No Hit
GTCTGCATCTGTGGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGA6790.10278472428262621No Hit
GATTTCACTCTCACCATCAGCAATCTGCAACCTGAAGATTTTGCAATTTATTACTGTCAACAGAGTTCCAATACC6730.10187646456878857No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACCGG400.00770004151.15212670
ATACACA3250.048.4386564
CTTATAC3200.047.116351
TATACAC3200.047.1020853
TCGTATG2800.046.7052240
TTATACA3250.046.384462
TATACTT307.298945E-444.6597565
ATCTCGT2700.044.28745737
ACGAGAC3450.043.73523722
AAGGGGG3850.042.91218669
CGTATGC3000.042.56688341
TACACAT3550.042.4582145
ACGTGAT659.901426E-441.97097870
GCCGTCT3350.041.8004946
CTTGAAA3650.041.76329457
CCCTAAG3600.041.1186328
TCTCCGA3700.040.73385211
ATCTCCG3750.040.1907410
TAAGACA3700.040.13547531
CTCGTAT3000.040.03489739
Produced by FastQC (version 0.11.5)