FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761448

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761448
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences660604
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG31220.47259780443351845No Hit
GTAATATACAGCCGTGTCTTCCCCTCTCAGGCTGTTCATTTGCAGATACA14700.22252362989022165No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT13430.2032987992806583No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT11970.18119781291060907No Hit
GTATATTACTGTGCGAGAAAGGGGTACAACCATGGTTACGATCGGGGCAATTGGGCAGAGTGGGACTACTTTGA11290.17090420282044916No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA11080.16772529382201742No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC10830.16394087834769394No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC10640.16106472258720808No Hit
GTTCCAATACCCCGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGA10300.1559179175421281No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG10230.15485828120931755No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC10020.1516793722108858No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC9760.14774358011758937No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT9650.14607843730888703No Hit
GTATTGGAACTCTGTTGACAGTAATAAATTGCAAAATCTTCAGGTTGCAGATTGCTGATGGTGAGAGTGAAAT9360.14168851535867177No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT8710.13184903512543067No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG8590.1300325156977554No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT8560.12957838584083656No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT8430.12761048979418835No Hit
GTGAGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGACACAGATTTCACT8360.1265508534613778No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG8210.12428020417678366No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG8090.12246368474910839No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT8020.12140404841629782No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG7970.12064716532143312No Hit
GTGCAAACTGGATGCAGCAAAGATCAGGAGTTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATTTAAATAG7970.12064716532143312No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG7730.11701412646608256No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA7640.11565173689532608No Hit
GTCTGCATCTGTGGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGA7560.11444072394354257No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC7560.11444072394354257No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG7320.11080768508819203No Hit
GATTTCACTCTCACCATCAGCAATCTGCAACCTGAAGATTTTGCAATTTATTACTGTCAACAGAGTTCCAATACC7280.11020217861230025No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTGGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTAGCAG7270.11005080199332731No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC7170.10853703580359791No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCTACAGCAAGCTCACCGTGGACAAGAG6990.10581225666208499No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC6880.10414711385338266No Hit
GTGGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTAGCAGCTATTTAAATTGGTATCAGCAGAAA6840.10354160737749091No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT6830.10339023075851797No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT6790.10278472428262621No Hit
GTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCCGGGGAGCGGGGGCTTGCCGGCCGTCGCA6770.10248197104468033No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT6750.10217921780673445No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG6700.10142233471186973No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC6650.10066545161700503No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAATATA307.2497607E-444.721091
GTATCAA17150.042.2438281
TATCAAC19050.037.998852
ATCAACG20050.035.7612273
CAACGCA20500.034.810155
TCAACGC20550.034.7280854
AACGCAG20600.034.641176
GTGGTAT2550.034.198481
CGTTTTA400.002968127433.5688123
GACGTTT400.0029736533.5560821
CGCAGAG22650.031.6513948
CGCCCAT652.4813173E-531.5344137
CGTGTCT4550.030.9396513
AGAGTAC23200.030.9150811
AATATAC4850.030.3965283
ATACAGT4650.030.05278646
CAGATAC4750.029.93344943
TCGCAAA500.005394888629.71071663
ATATACA4850.029.7056984
CCGTGTC4900.029.41371312
Produced by FastQC (version 0.11.5)