FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761449

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761449
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences795705
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACAGCCGTGTCCTCGGCTCTCAGGCTCTTCATTTGCAGAAACA29830.37488767822245683No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT23750.29847745081405797No Hit
GTGTATTACTGTGCGAAGGCGGGGGTCGTTCGAGGTTCGGGGAGTTATTGGTCAAGGAACGCTGGTGCGAATTA22490.2826424365813964No Hit
GTGTTGATGGTGAGAGTGAACTCTGTCCCAGATCCACTGCCACTGAACCT21990.27635870077478464No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG21110.2652993257551479No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC19410.243934624012668No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG18220.22897933279293203No Hit
CCATCAACACTCTGCAACCTGGAGATTTTGCAACTTACTACTGTCAACAG18120.2277225856316097No Hit
GAGTTCACTCTCACCATCAACACTCTGCAACCTGGAGATTTTGCAACTTACTACTGTCAACAGAGTTTCAGTGGC17410.218799680786221No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT16820.21138487253441915No Hit
CTCCAGCCCCGTCCCTGGAGCCTGGCGGACCCAGCCCATCCAATAGTTTC15950.20045117223091474No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACAATCACTTGCCGGGCAAGTCAGGGCATTAGCAA15940.20032549751478249No Hit
GTAGGAGACAGAGTCACAATCACTTGCCGGGCAAGTCAGGGCATTAGCAACTATTTAAATTGGTATCAGCAGAAA15750.19793767790827005No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG15210.19115124323712934No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC14880.1870039776047656No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT14820.18624992930797218No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT14670.18436480856598866No Hit
GTGCAGCCACAGTTCGTTTGACTTCCACCTTGGTCCCCTGGCCGAACGTC14620.1837364349853275No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC14200.17845809690777362No Hit
GATCTATGCAGCATCCACTTTGCAAAGTGAGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGAGTTCA14150.17782972332711242No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT14010.17607027730126115No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT13640.17142031280436845No Hit
GGGCTGGAGTGGGTGGCCAACATAAAACAAGATGGAAGTGAGAAACACTA13530.17003789092691388No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT13300.16714737245587247No Hit
GAGTGAACTCTGTCCCAGATCCACTGCCACTGAACCTTGATGGGACCTCA13240.16639332415907906No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGACTTCCACCTTGGTCCCCTGGCCGAACGTCCAAAGGCCA13130.16501090228162446No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG13080.1643825287009633No Hit
CAGTAATACACAGCCGTGTCCTCGGCTCTCAGGCTCTTCATTTGCAGAAA12600.158350142326616No Hit
GATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATTTAAAT12560.1578474434620871No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC12540.1575960940298226No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12520.15734474459755815No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC12450.1564650215846325No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT12130.152443430668401No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA12130.152443430668401No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG11860.14905021333283064No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG11480.1442745741198057No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC11420.1435205258230123No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT11310.1421381039455577No Hit
GCATAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATT11140.1400016337713097No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG11120.13975028433904524No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG11010.13836786246159066No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC10810.13585436813894597No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC10680.1342205968292269No Hit
GTAGTAAGTTGCAAAATCTCCAGGTTGCAGAGTGTTGATGGTGAGAGTGAACTCTGTCCCAGATCCACTGCCA10590.1330895243840368No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT10500.1319584519388467No Hit
CTCCTGTGCAGCCTCTGGATTCACGTTTAGAAACTATTGGATGGGCTGGGTCCGCCAGGCTCCAGGGACGGGG10490.13183277722271444No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG10370.13032468062912764No Hit
GCCTTAGCCCTGGACTCCAAGGCCTTTCCACTTGGTGATCAGCAGTGAGC10280.12919360818393752No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC10200.12818821045487963No Hit
GTCCCAGATCCACTGCCACTGAACCTTGATGGGACCTCACTTTGCAAAGT10200.12818821045487963No Hit
ACTTACTACTGTCAACAGAGTTTCAGTGGCCTTTGGACGTTCGGCCAGGGGACCAAGGTGGAAGTCAAACGAA10150.12755983687421846No Hit
GTCTGCATCTGTAGGAGACAGAGTCACAATCACTTGCCGGGCAAGTCAGG10100.12693146329355728No Hit
CTCCTGATCTATGCAGCATCCACTTTGCAAAGTGAGGTCCCATCAAGGTT10090.12680578857742505No Hit
GTCCAAAGGCCACTGAAACTCTGTTGACAGTAGTAAGTTGCAAAATCTCC9960.125172017267706No Hit
GTATTACTGTGCGAAGGCGGGGGTCGTTCGAGGTTCGGGGAGTTATTGGTCAAGGAACGCTGGTGCGAATTACTA9750.12253284822892907No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG9550.12001935390628436No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA9510.11951665504175543No Hit
GCCCTGGACTCCAAGGCCTTTCCACTTGGTGATCAGCAGTGAGCACAGAGGACTCACCATGGAATTGGGGCTGAG9490.11926530560949096No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT9310.11700316071911072No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA9290.11675181128684627No Hit
CAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCT9280.11662613657071402No Hit
CTATGTGGACTCTGTGGAGGGCCGATTCACCATCTCCAGAGACAACGCCA9280.11662613657071402No Hit
GCGTAGTAGTAGTAATTCGCACCAGCGTTCCTTGACCAATAACTCCCCGAACCTCGAACGACCCCCGCCTTCGCA9160.1151180399771272No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC8920.11210184678995357No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT8910.11197617207382132No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC8770.11021672604797003No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC8670.10895997888664768No Hit
GTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACAATCA8560.10757755700919311No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCAG8490.10669783399626746No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT8450.1061951351317385No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT8430.10594378569947405No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCTACAGCAAGCTCACCGTGGACAAGAG8370.10518973740268064No Hit
ATGCAGCATCCACTTTGCAAAGTGAGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGAGTTCACTCTCA8330.1046870385381517No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCC8260.10380731552522605No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA8200.10305326722843265No Hit
CTACTACGCTGTGGACGTCTGGGGCCAAGGGACCACGGTCACCGTCTCCTCAGCCTCCACCAAGGGCCCATCGGT8200.10305326722843265No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT8160.1025505683639037No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG8140.10229921893163924No Hit
GTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCCGGGGAGCGGGGGCTTGCCGGCCGTCGCA8110.10192219478324252No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG8020.10079112233805242No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC8010.10066544762192019No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATAGC150.00229129969.4808247
TCGTATG700.059.2464140
CGTATGC750.055.3316141
CTTATAC850.052.584631
ATACGCT200.007342223551.77844638
ACGAGAC850.048.5366122
GTGGTAT1650.047.926791
TCTGGAG9800.047.20345770
CTCGTAT901.8189894E-1242.21915439
TTATACA1250.041.256122
CCCACGA1000.041.24315619
GTATCAA14700.040.697371
TATACAC1100.040.620813
ATCTCGT955.456968E-1239.95167537
GATCTCG955.456968E-1239.93404436
CACGAGA1050.039.28413421
TACACAT1400.039.2841345
TATTGGC459.160326E-538.9168557
GTATGCC1200.037.49730742
CGAGACA1200.037.24744823
Produced by FastQC (version 0.11.5)