FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761453

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761453
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1076459
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAAAGCCTCGTA43930.40809728935333345No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT40060.37214608266548005No Hit
GTACATATTGGCCCTTGTACACCTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT31430.291975820723316No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC31010.2880741393773474No Hit
CCTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA29490.2739537687919373No Hit
GGCGTAGACTTTGTGCTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC29140.27070236767029676No Hit
GTCCAAGGGTGACGGGCAGGGAGATTGGAGCCTGAATCATCACAACATCC26470.24589882197092505No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG26280.2441337756477488No Hit
CTATTACTGCATGCAAGGTACATATTGGCCCTTGTACACCTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA24640.2288986389634905No Hit
CAGTAATAGACCCCAACATCCTCAGCCTCCACCCTGCTGATTTTCAGTGTGAAGTCAGTGCCTGACCCACTGCCG24530.22787677003954632No Hit
GTAATAGACCCCAACATCCTCAGCCTCCACCCTGCTGATTTTCAGTGTGAAGTCAGTGCCTGACCCACTGCCG24350.22620462089127405No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC24150.2243466773931938No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG23180.21533565142750444No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTC22720.2110623813819198No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT22640.2103192039826877No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA22480.20883284918422346No Hit
GTCTGGGACCCCAGAGTCCCGATTAAAAACCTTATAAATCAGGCGCCTTGGAGATTGGCCTGGCCTCTGGTGAAT22410.20818256895989537No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG20490.19034631137832467No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG20310.18867416223005243No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20170.18737360178139623No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT19390.18012762213888314No Hit
GCTAATGCTCTGGGTCCCAGGATCCAGTGGGGATGTTGTGATGATTCAGG19100.17743360406666672No Hit
GGCCAAAGGTGTACAAGGGCCAATATGTACCTTGCATGCAGTAATAGACCCCAACATCCTCAGCCTCCACCCTG18980.17631883796781855No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCCCA18880.17538986621877842No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATCCAGTGGGGATGTTGTGATGATTCAGGCTCCAAT18230.16935154985001752No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG18000.1672149148272252No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATCCAGTGGGGATGTTGTGATGATTCAGGCTCCAATCTCC17930.16656463460289708No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT17850.16582145720366498No Hit
GTCCCAGACAGATTCAGCGGCAGTGGGTCAGGCACTGACTTCACACTGAA17750.16489248545462484No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG17420.16182687868279239No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG17060.15848258038624788No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC16710.1552311792646074No Hit
ATATTGGCCCTTGTACACCTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTT16540.15365192729123917No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT16540.15365192729123917No Hit
GTCTATTACTGCATGCAAGGTACATATTGGCCCTTGTACACCTTTGGCCA16400.15235136684258294No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACA16370.15207267531787091No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAGGTG16070.14928576007075048No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT15750.14631305047382204No Hit
GGCCAATATGTACCTTGCATGCAGTAATAGACCCCAACATCCTCAGCCTC15550.14445510697574176No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC15350.1425971634776615No Hit
GTGATGGAAACACCTACTTGAGTTGGATTCACCAGAGGCCAGGCCAATCT15310.14222557477804543No Hit
AATATGTACCTTGCATGCAGTAATAGACCCCAACATCCTCAGCCTCCACC14910.13850968778188485No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14770.13720912733322868No Hit
CCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCT14610.13572277253476445No Hit
ATATGTACCTTGCATGCAGTAATAGACCCCAACATCCTCAGCCTCCACCC14410.13386482903668417No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGCTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG14080.13079922226485172No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA14030.13033473639033163No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG13830.12847679289225136No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG13660.1268975409188831No Hit
GGTCTAGTCAAAGCCTCGTACATAGTGATGGAAACACCTACTTGAGTTGGATTCACCAGAGGCCAGGCCAATCTC13510.1255040832953229No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT13040.12113791607483425No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA12890.11974445845127403No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT12870.11955866410146603No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG12390.11509959970607334No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA12150.112870067508377No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC12030.11175530140952884No Hit
GCCTCGTACATAGTGATGGAAACACCTACTTGAGTTGGATTCACCAGAGG11550.10729623701413615No Hit
GTACGAGGCTTTGACTAGACCTGCAGGAGATGGAGGCCGGCTGTCCAAGGGTGACGGGCAGGGAGATTGGAGC11120.10330165849326356No Hit
GGCACTGACTTCACACTGAAAATCAGCAGGGTGGAGGCTGAGGATGTTGG11020.10237268674422341No Hit
GTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAAT10920.10144371499518329No Hit
GAGCAAAGCAGACTACGAGAAGCACAAAGTCTACGCCTGCGAAGTCACCC10890.10116502347047125No Hit
TCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATCCAGTGGGGATGTTGTGATGATTCAGGCTC10860.10088633194575919No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA10780.1001431545465271No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC2650.047.4697232
GTGGTAT2700.046.5928151
GGTATCA8750.046.2402231
GTATCAA22900.041.1268351
CTTATAC1300.036.6155621
TATCAAC26500.035.666442
ATCAACG28100.033.509953
TCAACGC28550.032.981774
CAACGCA28600.032.9241075
AACGCAG28750.032.7523356
AATGGTA2800.032.56445370
GCTTGAC951.0139047E-832.210681
ACGCAGA30500.030.87317
TATACAC1550.030.7041173
CGCAGAG31250.030.1321458
AGAGTAC31100.030.05886711
TCGTATG1151.7334969E-929.95078840
CAGAGTA31450.029.72434810
GTGTATA1151.9954314E-929.55986613
GCAGAGT32000.029.3196939
Produced by FastQC (version 0.11.5)