FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761454

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761454
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1076459
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT40070.37223897984038407No Hit
CCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAAAGCCTCGTA36830.34214029517148353No Hit
GGCGTAGACTTTGTGCTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC30510.28342928063214673No Hit
GTACATATTGGCCCTTGTACACCTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT28950.2689373213471205No Hit
CCTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA27200.252680315738918No Hit
GTCCAAGGGTGACGGGCAGGGAGATTGGAGCCTGAATCATCACAACATCC25440.23633041295581161No Hit
CAGTAATAGACCCCAACATCCTCAGCCTCCACCCTGCTGATTTTCAGTGTGAAGTCAGTGCCTGACCCACTGCCG25040.23261452595965104No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG24510.2276909756897383No Hit
GTAATAGACCCCAACATCCTCAGCCTCCACCCTGCTGATTTTCAGTGTGAAGTCAGTGCCTGACCCACTGCCG23490.21821546384952886No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC23470.21802966949972086No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC23030.21394219380394425No Hit
CTATTACTGCATGCAAGGTACATATTGGCCCTTGTACACCTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA22620.21013340963287963No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT22570.20966892375835958No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTC21540.20010051474324614No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA21040.19545565599804546No Hit
GTCTGGGACCCCAGAGTCCCGATTAAAAACCTTATAAATCAGGCGCCTTGGAGATTGGCCTGGCCTCTGGTGAAT20420.18969603115399658No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19040.17687622101724265No Hit
GCTAATGCTCTGGGTCCCAGGATCCAGTGGGGATGTTGTGATGATTCAGG18970.17622594079291454No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG18460.17148818487280984No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG18100.16814388657626533No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT18010.1673078120021292No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17890.16619304590328102No Hit
GGCCAAAGGTGTACAAGGGCCAATATGTACCTTGCATGCAGTAATAGACCCCAACATCCTCAGCCTCCACCCTG17650.16396351370558468No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT17620.16368482218087266No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT17250.16024762670942416No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG17000.15792519733682378No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCCCA16840.15643884253835957No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATCCAGTGGGGATGTTGTGATGATTCAGGCTCCAAT16660.1547666933900873No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACA16500.1532803385916231No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG16260.15105080639392676No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG16210.15058632051940668No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATCCAGTGGGGATGTTGTGATGATTCAGGCTCCAATCTCC16210.15058632051940668No Hit
GTCCCAGACAGATTCAGCGGCAGTGGGTCAGGCACTGACTTCACACTGAA16050.14909996572094247No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG16040.14900706854603846No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA15940.14807809679699832No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAGGTG15860.1473349193977662No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC15620.14510538720006985No Hit
ATATTGGCCCTTGTACACCTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTT15350.1425971634776615No Hit
GTCTATTACTGCATGCAAGGTACATATTGGCCCTTGTACACCTTTGGCCA15180.14101791150429324No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG15150.1407392199795812No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG15070.1399960425803491No Hit
GGCCAATATGTACCTTGCATGCAGTAATAGACCCCAACATCCTCAGCCTC15040.13971735105563704No Hit
AATATGTACCTTGCATGCAGTAATAGACCCCAACATCCTCAGCCTCCACC14900.13841679060698087No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC14480.13451510926101226No Hit
ATATGTACCTTGCATGCAGTAATAGACCCCAACATCCTCAGCCTCCACCC13340.12392483132195467No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA13300.12355324262233862No Hit
GTGATGGAAACACCTACTTGAGTTGGATTCACCAGAGGCCAGGCCAATCT12980.12058053302541018No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT12920.12002314997598608No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGCTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG12820.11909417822694593No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAGCACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT12760.11853679517752186No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG12610.11714333755396165No Hit
GTCAGTGCCTGACCCACTGCCGCTGAATCTGTCTGGGACCCCAGAGTCCCGATTAAAAACCTTATAAATCAGGCG12450.11565698275549743No Hit
GGTCTAGTCAAAGCCTCGTACATAGTGATGGAAACACCTACTTGAGTTGGATTCACCAGAGGCCAGGCCAATCTC12400.11519249688097734No Hit
CCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCT12370.1149138053562653No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT12140.112777170333473No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11960.11110502118520074No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG11770.10933997486202447No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAGCACAAAGTCTACGCCTGCGAAGTCA11730.1089683861624084No Hit
GTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAAT11390.10580988221567193No Hit
GTGCCTGACCCACTGCCGCTGAATCTGTCTGGGACCCCAGAGTCCCGATT11300.10497380764153581No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA10830.10060764042104715No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGAT250.001252043880.90790670
AAGTATA554.6038636E-449.03508870
GTGGTAT3350.044.6114921
GGTATCA9500.041.4737471
TACACGC451.1033646E-437.707965
TGGTATC4150.037.6188432
GTATCAA22400.037.6047361
ATACCAT502.0501942E-433.937166
TATCAAC24900.033.393542
TTTGCGA450.003394826832.66265566
GCTCGGG3100.032.62415370
ATCAACG25500.032.6002353
ACATATG2900.032.54915670
TCAACGC25750.032.1579324
CAACGCA25850.032.033535
AACGCAG25850.032.033536
TGTTAGA11000.031.25987270
ACGCAGA26950.030.8519677
AGAGTAC28150.029.41759311
CAGAGTA28350.029.20870610
Produced by FastQC (version 0.11.5)