FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761461

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761461
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2172858
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22000.039.758271
GTATCAA52000.037.797341
TCGTATG3200.035.68362440
TCAACGC59450.033.114644
CAACGCA59650.032.9461145
TATCAAC60550.032.7970242
ATCAACG60600.032.5420723
AACGCAG61750.031.7672126
GTGGTAT8550.030.0888161
ACGCAGA67200.029.1908557
CGCAGAG68900.028.3710678
AGAGTAC69750.027.97551511
CAGAGTA70700.027.69725210
GCAGAGT75850.025.86199
TGGTATC9700.025.8119682
AGTACGG56400.024.3220913
CGTATGC4750.024.06239941
GAGTACG57150.024.00235212
TCTCGTA4700.023.51751538
CTTATAC6200.023.2363321
Produced by FastQC (version 0.11.5)