FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761462

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761462
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2172858
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG31650.14566069204706428No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21550.039.4697231
GTATCAA52200.038.568011
TCAACGC59900.033.3312154
CAACGCA59950.033.2447625
TATCAAC60550.033.0987172
ATCAACG60250.033.0784653
AACGCAG61200.032.509796
GTGGTAT8950.032.1896251
ACGCAGA64250.030.9665227
CGCAGAG65200.030.5685488
AGAGTAC68050.029.24336811
CAGAGTA69000.028.88572910
TGGTATC9500.028.4887142
GCAGAGT71400.027.9141399
AGTACGG62750.025.76485313
GAGTACG63500.025.46054512
GTACGGG68750.023.2173514
TACGGGG28100.021.94247415
TACGGGA23550.021.81824115
TACGGGT10650.019.62002815
Produced by FastQC (version 0.11.5)