FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761463

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761463
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences714061
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGGGGCCTGGCTTGGCCTCTGCTGGTACCAATAAGCATATTGCTTT16200.2268713737341768No Hit
GACGTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACTCTCCGAAAAGTG13910.1948012844840987No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT13580.19017983057469878No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG13230.1852782885495777No Hit
GCCTTGGGCTGACGTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACTCTCCGAAAAGTGCCACTGCTGTCTG13130.18387784797097168No Hit
CTATTACTGTCAGTCAGCAGACAGCAGTGGCACTTTTCGGAGAGTGTTCG12560.1758953366729173No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCAGCTTGGTCCCTC12420.17393471986286885No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG12420.17393471986286885No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG12320.17253427928426282No Hit
GTCCAGGAGGCAGAACTCTGGGTGTCTCACCATGGCCTGGATCCCTCTAC11710.16399159175476605No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT11550.16175088682899638No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT11430.16007035813466916No Hit
GTGTCAGTGTCCCCGGGACAGACGGCCAGGATCACCTGCTCTGGAGATGC11040.15460863987810564No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT11000.15404846364666325No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC10950.15334824335736022No Hit
CTCTCTGCACAGGCTCTGAGGCCTCCTATGAGCTGACACAGCCGCCCTCGGTGTCAGTGTCCCCGGGACAGACGG10810.15138762654731178No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG10340.14480555582786345No Hit
TCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG10310.14438542365428164No Hit
CTCCAGCCCCTTGCCTGGAGCCTGGCGGACCCAGTGCATGCCATAAGTCC10160.1422847627863726No Hit
GGCTTGGCCTCTGCTGGTACCAATAAGCATATTGCTTTGGCAATGCATCTCCAGAGCAGGTGATCCTGGCCGT9980.13976396974488176No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT9890.1385035732241363No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA9840.1378033529348333No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC9820.1375232648191121No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA9810.13738322076125148No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC9720.13612282424050606No Hit
GTCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC9640.13500247177762123No Hit
TCAGTGGAGTCCGGGCAGAGGACGAGGGTGACTATTACTGTCAGTCAGCA9560.13388211931473643No Hit
CAGTAATAGTCACCCTCGTCCTCTGCCCGGACTCCACTGATGGTCAACGT9420.13192150250468798No Hit
GGCTCTGAGGCCTCCTATGAGCTGACACAGCCGCCCTCGGTGTCAGTGTCCCCGGGACAGACGGCCAGGATCAC9410.13178145844682737No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG9350.13094119409966376No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT9330.13066110598394257No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCAGCTTGGTCCCTCCGCCGAACA9260.12968079757891832No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA8970.12561951990096085No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC8930.12505934366951843No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG8860.12407903526449422No Hit
GGACGAGGGTGACTATTACTGTCAGTCAGCAGACAGCAGTGGCACTTTTC8760.12267859468588818No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC8620.12071797787583975No Hit
CAATAATACACGGCCGTGTCCTCAATTCTCAGGCCGTTCATTTGCAGATA8580.12015780164439734No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC8490.11889740512365189No Hit
CCTATGAGCTGACACAGCCGCCCTCGGTGTCAGTGTCCCCGGGACAGACGGCCAGGATCACCTGCTCTGGAGATG8260.11567639179285802No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT8160.11427595121425199No Hit
CTGCTGGTACCAATAAGCATATTGCTTTGGCAATGCATCTCCAGAGCAGGTGATCCTGGCCGTCTGTCCCGGGGA7850.10993458542057331No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC7680.10755383643694306No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG7610.10657352803191884No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC7610.10657352803191884No Hit
GGCCAAGCCAGGCCCCTATACTATTAATATATGAGGACACTGAGAGGCCC7600.10643348397405823No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA7520.10531313151117341No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA7440.1041927790482886No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG7290.10209211818037955No Hit
ACGTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACTCTCCGAAAAGTGC7290.10209211818037955No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTC7260.10167198600679775No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC7180.1005516335439129No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG7170.10041158948605232No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGGTA252.5864947E-455.11345717
GGTATCA4900.042.8846441
TGGTATC1600.040.907382
GTATCAA11600.038.3089071
GTGGTAT1700.036.474691
CGAACTA601.3511382E-534.46521429
TAGGAAC400.0026148334.4483224
TATCAAC12800.034.179192
ATCAACG13550.032.2873543
TCAACGC13650.032.0508164
CAACGCA13850.031.587995
CAACTCG450.004136238231.36982565
TATGTAT450.004136238231.36982565
AACGCAG14150.030.9161156
CGTAGTC450.00464757530.63145628
CGCAGAG14600.029.963228
AGAGTAC15000.028.9345611
CAGAGTA15150.028.87544610
CAACGGA755.3407908E-528.18431564
GCGAACT756.208681E-527.56830828
Produced by FastQC (version 0.11.5)