FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761467

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761467
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences167103
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT1830.1095132941957954No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCAGAT253.75491E-451.0826719
AATGGGG500.00871052349.53575570
TATTAGA353.5867033E-545.595882
TTTAACG308.3845994E-443.40026534
ATTAGAG309.236299E-442.5561563
ACGATTA350.001024865541.66272750
GTATCAA2600.040.5101851
GTATTGG350.001970536536.4767041
TCCCGAT451.577415E-435.46346311
TTCAACG400.002433820634.93842346
TCAACGC3100.032.94674
TCCTACG500.003355173932.7199460
CGATTAA450.00340333732.6273651
ATCAACG3150.032.4237373
AACGATT450.003669532232.12982649
GCTATTA502.8574723E-432.05138427
CGCTATT502.8726284E-432.02251426
TACGGGC701.7720504E-631.92666815
GATCAGG801.393073E-731.9171161
ATCCCGA502.9287708E-431.91711610
Produced by FastQC (version 0.11.5)