FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761470

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761470
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1192308
Sequences flagged as poor quality0
Sequence length20-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16030.13444512659480604No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA35950.039.9413531
GGTATCA17700.034.5742031
TCAACGC42650.033.7003144
TATCAAC42500.033.674132
ATCAACG43350.033.2320983
CAACGCA43600.032.887715
AACGCAG45100.031.7181846
TCGTACG450.00483088230.39179623
CGCAGAG47800.029.995488
ACGCAGA48250.029.7182247
GTGGTAT5100.029.4954411
AGAGTAC48750.029.20332511
CAGAGTA49800.028.72229410
AGTACGG39850.027.75796113
GCAGAGT52350.027.3884229
GAGTACG40800.027.11163312
TACGGGT10600.026.74056615
TACGGAT1801.4298947E-524.4277270
GTACGGG44800.024.3109414
TACGGGG16300.023.88447215
Produced by FastQC (version 0.11.5)