FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761471

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761471
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences650185
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCATAGGACTGGCAGTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAG20100.3091427824388443No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA17950.2760752708844406No Hit
GTCCTATGACAGTCGCCTGACTGGTTGGGTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGTCAGCCCAAGGC16280.2503902735375316No Hit
CTCCTGCACTGGGAGCAGCTCCAACATCGGGGCAGGTTATGATGTACACTGGTATCAGCAGCTTCCAGGAACAGC16080.24731422595107547No Hit
GACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACCCAACCAGTCAGG15640.2405469212608719No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT15360.23624045463983326No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG14840.22824273091504726No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC14540.223628659535363No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC13230.20348054784407515No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC12310.1893307289463768No Hit
GGTTATGATGTACACTGGTATCAGCAGCTTCCAGGAACAGCCCCCAAACTCCTCATCTATGGTAACAGCGATCGG12060.18548566946330658No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC11880.18271722663549606No Hit
GTCCTGGGCCCAGTCTGTGCTGACGCAGGCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCACCATCTCCTG11050.16995162915170298No Hit
ACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACCCAACCAGTCAGGC10940.16825980297915208No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACCCAACCAGTCAGGCGACTGTCATAGGA10810.1662603720479556No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC10610.16318432446149944No Hit
GGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGCCCAGT10160.15626321739197307No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT9860.15164914601228882No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG9710.14934211032244668No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC9560.14703507463260457No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTC9250.1422672008735975No Hit
GGCCATCACTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCCAGT9230.1419595961149519No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT9130.1404215723217238No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG8840.1359613033213624No Hit
GTGCTGACGCAGGCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCAC8730.1342694771488115No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG8640.13288525573490623No Hit
GATTATTACTGCCAGTCCTATGACAGTCGCCTGACTGGTTGGGTCTTCGG8410.12934780101048163No Hit
GTGATGGCCAGGGAGGCTGAGGTGTCAGACTTGGAGCCAGAGAATCGGTCAGGGACCCCCGAGGGCCGATCG8380.12888639387251322No Hit
GCCCTCGGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGACACCTCAG8180.12581034628605703No Hit
ATGGTAACAGCGATCGGCCCTCGGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGACACCTCAGCCTCCCTGG8050.12381091535486052No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT7900.12150387966501841No Hit
GGGCAGAGGGTCACCATCTCCTGCACTGGGAGCAGCTCCAACATCGGGGCAGGTTATGATGTACACTGGTATCAG7880.12119627490637279No Hit
GATGTACACTGGTATCAGCAGCTTCCAGGAACAGCCCCCAAACTCCTCAT7670.11796642494059384No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG7470.11489037735413768No Hit
GTCTGACACCTCAGCCTCCCTGGCCATCACTGGGCTCCAGGCTGAGGATG7350.11304474880226398No Hit
GTGTCTGGGGCCCCAGGGCAGAGGGTCACCATCTCCTGCACTGGGAGCAG7300.11227573690564994No Hit
CTGTTACCATAGATGAGGAGTTTGGGGGCTGTTCCTGGAAGCTGCTGATA7050.10843067742257974No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA7000.10766166552596569No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC6890.1059698393534148No Hit
AGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGT6880.10581603697409198No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA6810.10473942031883232No Hit
GCACAGGGTCCTGGGCCCAGTCTGTGCTGACGCAGGCGCCCTCAGTGTCT6600.10150957035305337No Hit
CTTCAGCTGTGGGCACAAGAGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTC6560.10089436083576211No Hit
CTGCACAGGGTCCTGGGCCCAGTCTGTGCTGACGCAGGCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCAC6550.10074055845643932No Hit
TCACTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCCAGTCCTAT6530.10043295369779372No Hit
GTGCAGGAGATGGTGACCCTCTGCCCTGGGGCCCCAGACACTGAGGGCGC6510.10012534893914808No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTTCG150.00239364368.7179915
CGAATAT200.00745272651.5821330
GTATCAA14400.043.1906661
ATCAACG16800.037.4296383
TCAACGC16800.037.426764
TATCAAC16650.037.354092
CAACGCA17300.036.3450625
AACGCAG17950.035.0289466
AGAGTAC18600.033.80481711
CGCAGAG19100.032.9198768
CAGAGTA19300.032.57873510
CTAGCGC652.3733295E-531.74040428
GCGTAGG1053.1488255E-431.53685670
ACGCAGA20000.031.4384787
GCAGAGT20150.031.374969
TATCTAG553.3331037E-431.2378521
GTGGTAT1800.030.5436761
CCCTAGA853.599222E-529.86147969
CGTATGC703.8023278E-529.62371341
CTCGTAT703.836585E-529.58480639
Produced by FastQC (version 0.11.5)