FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761473

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761473
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences844907
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAATCAGCCTCATCCTCGGACCGGAGCCCACTGATGGCCAGGGAGG67710.8013899754647552No Hit
GATTATTACTGTGCAGCATGGGATGACAATTTGAGTGGTTGGGTGTTCGGCGGAGGGACTAAATTGACCGTCCTA56160.6646885396854328No Hit
GACGTAGGACGGTCAATTTAGTCCCTCCGCCGAACACCCAACCACTCAAA31610.3741240160159639No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAACGTCTGCGACCC30290.3585009947840413No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT28640.3389722182441381No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCAATTTAGTCCCTC27360.3238226218980314No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT26620.3150642615104384No Hit
GCCTTGGGCTGACGTAGGACGGTCAATTTAGTCCCTCCGCCGAACACCCAACCACTCAAATTGTCATCCCATG26180.30985658776646424No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGG26000.307726175780293No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAACGTCT25910.30666096978720736No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC25840.30583247623702964No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG24760.29305000432000206No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG24590.29103794855528475No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG24280.28736890569021206No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA23240.27505985865900034No Hit
TCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG23150.2739946526659147No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT22450.2657097171641376No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC22350.26452615494959797No Hit
GTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCA22330.26428944250669006No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG21960.2599102623128936No Hit
GTGCTGACTCAGCCACCCTCAACGTCTGCGACCCCCGGACAGAGGGTCAC21910.25931848120562384No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC20750.24558915951696458No Hit
CCCTCAACGTCTGCGACCCCCGGACAGAGGGTCACCATCTCTTGTTCTGG20510.24274861020206956No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT20140.23836943000827307No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC19980.23647573046500975No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG19950.23612066180064786No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA19170.22688887652723908No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA19140.22653380786287725No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG18670.22097106545454115No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG18170.21505325438184322No Hit
CTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTC18030.21339626728148778No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC17570.20795188109460566No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA16750.19824667093538106No Hit
GTGCAGCATGGGATGACAATTTGAGTGGTTGGGTGTTCGGCGGAGGGACT16720.19789160227101915No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCAATTTAGTCCCTCCGCCGAACAC16670.19729982116374936No Hit
GTCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC16660.19718146494229544No Hit
ATTTAGTCCCTCCGCCGAACACCCAACCACTCAAATTGTCATCCCATGCTGCACAGTAATAATCAGCCTCATCCT16590.19635297139211771No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC16550.19587954650630185No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA16460.19481434051321625No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC16090.1904351603194198No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC16000.18936995432633413No Hit
ATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC15390.18215022481764265No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA15170.17954638794565556No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCAATTTAGTC15040.1780077570667541No Hit
GGCCATCAGTGGGCTCCGGTCCGAGGATGAGGCTGATTATTACTGTGCAG14800.1751672077518591No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC14470.17126145244387844No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT14440.17090638377951656No Hit
ATGCTGCACAGTAATAATCAGCCTCATCCTCGGACCGGAGCCCACTGATG14270.16889432801479926No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCGGTCCGAGGATG14190.16794747824316758No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGG14140.1673556971358978No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT14120.16711898469298989No Hit
GTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGC13920.1647518602639107No Hit
GTCAGGGACCCCGGAGGACCGCTGATTATTCCTATAGATGAGTAGTTTGG13870.1641600791566409No Hit
GTCCGAGGATGAGGCTGATTATTACTGTGCAGCATGGGATGACAATTTGAGTGGTTGGGTGTTCGGCGGAGGGA13850.16392336671373298No Hit
GCATGGGATGACAATTTGAGTGGTTGGGTGTTCGGCGGAGGGACTAAATTGACCGTCCTACGTCAGCCCAAGG13750.1627398044991934No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTG13710.1622663796133776No Hit
GTGCCAGACTTGGAGCCAGAGAATCGGTCAGGGACCCCGGAGGACCGCTG13700.1621480233919236No Hit
GGACTAAATTGACCGTCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAG13210.15634856854067963No Hit
GAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCT13180.15599349987631775No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG13110.15516500632614003No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT13040.15433651277596233No Hit
GTTTGGGGGCCGTTGGGGCCGCTCCTGGAAATTGCTGGTACCAGTCTACATAATTATTGCCGATGTTGGATCTG12600.14912883903198815No Hit
CCCCCGGACAGAGGGTCACCATCTCTTGTTCTGGAAGCAGATCCAACATC12600.14912883903198815No Hit
CCCCGGAGGACCGCTGATTATTCCTATAGATGAGTAGTTTGGGGGCCGTT12550.14853705792471833No Hit
GTCCTCCGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAG12460.14747185193163273No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC12430.14711678326727085No Hit
TCCTCGGACCGGAGCCCACTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTCAGGGACC12410.1468800708243629No Hit
ATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTG12210.14451294639528373No Hit
CCTCCGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCGGT12170.1440395215094679No Hit
CGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC12120.14344774040219813No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG12030.14238253440911247No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC11940.14131732841602684No Hit
ACGTAGGACGGTCAATTTAGTCCCTCCGCCGAACACCCAACCACTCAAAT11700.13847677910113185No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA11640.13776664177240808No Hit
CTCTTGTTCTGGAAGCAGATCCAACATCGGCAATAATTATGTAGACTGGT11450.13551787356478287No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG11210.13267732424988787No Hit
CTCAAATTGTCATCCCATGCTGCACAGTAATAATCAGCCTCATCCTCGGA11170.13220389936407204No Hit
GTAATAGACAGCCGTGTCCTCACGTCTCGGGAGGTCCATTTGCAAAAATA11160.13208554314261808No Hit
GCACAGTAATAATCAGCCTCATCCTCGGACCGGAGCCCACTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAG11130.13173047447825617No Hit
GTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCA11060.13090198092807848No Hit
CTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTG11040.13066526848517057No Hit
GATTATTCCTATAGATGAGTAGTTTGGGGGCCGTTGGGGCCGCTCCTGGA10890.12888992516336117No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG10710.12675951317718992No Hit
GTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTC10650.12604937584846618No Hit
TTACTGTGCAGCATGGGATGACAATTTGAGTGGTTGGGTGTTCGGCGGAG10620.1256943071841043No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA10550.12486581363392657No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC10490.12415567630520283No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGC10440.12356389519793302No Hit
GGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGT10420.12332718275502512No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG10340.12238033298339343No Hit
GTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCT10260.12143348321176177No Hit
GCCCCAACGGCCCCCAAACTACTCATCTATAGGAATAATCAGCGGTCCTCCGGGGTCCCTGACCGATTCTCTGG10250.12131512699030782No Hit
GTCTATTACTGTGCCAAAGATCTAGGAGGATACGCTTATGGTTTTGAAAGACTTGATTCTTACGGTCTGGACGT10110.11965813988995239No Hit
ATCCCATGCTGCACAGTAATAATCAGCCTCATCCTCGGACCGGAGCCCAC10080.11930307122559052No Hit
GCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC10030.11871129011832073No Hit
TCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAA10000.11835622145395883No Hit
GGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTG9920.11740937168232717No Hit
ACATAATTATTGCCGATGTTGGATCTGCTTCCAGAACAAGAGATGGTGAC9850.11658087813214947No Hit
GATGAGTAGTTTGGGGGCCGTTGGGGCCGCTCCTGGAAATTGCTGGTACC9720.11504224725324798No Hit
CCGTTGGGGCCGCTCCTGGAAATTGCTGGTACCAGTCTACATAATTATTG9700.11480553481034007No Hit
GGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCAATTTAGTCCCTCCGCCGAACACC9660.11433210992452424No Hit
CAATTTGAGTGGTTGGGTGTTCGGCGGAGGGACTAAATTGACCGTCCTACGTCAGCCCAAGGCTGCCCCCTCGGT9540.11291183526707672No Hit
CCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGG9530.11279347904562277No Hit
AGTCTGTGCTGACTCAGCCACCCTCAACGTCTGCGACCCCCGGACAGAGG9420.11149156060962923No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCT9410.11137320438817527No Hit
CCTCAGCCTCCCTGGCCATCAGTGGGCTCCGGTCCGAGGATGAGGCTGAT9370.11089977950235944No Hit
GGTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGG9350.11066306705945152No Hit
GTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTT9260.10959786106636588No Hit
TCAGTGGGCTCCGGTCCGAGGATGAGGCTGATTATTACTGTGCAGCATGG9250.10947950484491192No Hit
GATGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG9160.10841429885182628No Hit
CTCCTGGAAATTGCTGGTACCAGTCTACATAATTATTGCCGATGTTGGATCTGCTTCCAGAACAAGAGATGGTGA9080.10746744908019462No Hit
GCCTCATCCTCGGACCGGAGCCCACTGATGGCCAGGGAGGCTGAGGTGCC9080.10746744908019462No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC9060.10723073663728672No Hit
GGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCC9040.10699402419437878No Hit
GGGTCACCATCTCTTGTTCTGGAAGCAGATCCAACATCGGCAATAATTAT9020.10675731175147088No Hit
GGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGG8990.10640224308710898No Hit
TAATTATGTAGACTGGTACCAGCAATTTCCAGGAGCGGCCCCAACGGCCC8880.10510032465111543No Hit
GGATGACAATTTGAGTGGTTGGGTGTTCGGCGGAGGGACTAAATTGACCGTCCTACGTCAGCCCAAGGCTGCCCC8870.10498196842966148No Hit
GGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCT8820.10439018732239169No Hit
CTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTC8800.10415347487948377No Hit
TCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGG8750.10356169377221398No Hit
CCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTT8690.10285155644349023No Hit
CTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAACGTCTGCGACCCCCGGACAGAGGGTCAC8650.10237813155767439No Hit
GAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAG8610.10190470667185857No Hit
CTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCT8600.10178635045040461No Hit
ATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCC8560.10131292556458876No Hit
CAGTAATAATCAGCCTCATCCTCGGACCGGAGCCCACTGATGGCCAGGGA8550.10119456934313481No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGGCGC406.992159E-753.01740365
CTTATAC750.050.4736751
TATACAC750.050.458743
GGTATCA5500.048.8051261
AGGGGGG1359.094947E-1248.71405470
GTGGTAT1550.046.6252441
TTGTATC306.3924375E-445.8797317
TCGTATG704.3291948E-1044.4648740
CGAGACA803.45608E-1143.0147923
GTATCAA14100.042.224111
TCTGGAG5200.040.47013570
CGTATGC801.5952537E-938.9229741
TATCAAC16200.036.746262
ACGAGAC1051.6370905E-1136.05048822
TTATACA1051.6370905E-1136.0483552
GCCAGAG28950.035.89190770
ACGTCTC3050.034.9759622
TCTCGTA807.0027454E-834.5488538
TGGTATC2000.034.4097942
TACACAT1003.929017E-1034.4036875
Produced by FastQC (version 0.11.5)