FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761474

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761474
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences844907
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTATTACTGTGCAGCATGGGATGACAATTTGAGTGGTTGGGTGTTCGGCGGAGGGACTAAATTGACCGTCCTA53450.63261400367141No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAACGTCTGCGACCC39070.4624177572206172No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAACGTCT30290.3585009947840413No Hit
GTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCA30100.3562522265764161No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGG29620.3505711279466261No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG28220.33400125694307187No Hit
GTGCTGACTCAGCCACCCTCAACGTCTGCGACCCCCGGACAGAGGGTCAC27690.327728377206012No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT26910.31849659193260327No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC25940.3070160384515692No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG25240.2987311029497921No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC25220.2984943905068842No Hit
GACGTAGGACGGTCAATTTAGTCCCTCCGCCGAACACCCAACCACTCAAA24690.2922215107698244No Hit
CCCTCAACGTCTGCGACCCCCGGACAGAGGGTCACCATCTCTTGTTCTGG23200.2745864337731845No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT23140.27387629644446077No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCAATTTAGTCCCTC22550.2668932793786772No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA22120.26180396185615695No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG22110.261685605634703No Hit
GTAATAATCAGCCTCATCCTCGGACCGGAGCCCACTGATGGCCAGGGAGG21900.25920012498416983No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC21760.25754313788381444No Hit
GCCTTGGGCTGACGTAGGACGGTCAATTTAGTCCCTCCGCCGAACACCCAACCACTCAAATTGTCATCCCATG21550.2550576572332813No Hit
TCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG21400.25328231391147193No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG20480.24239354153770767No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG19220.22748065763450886No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCAATTTAGTCCCTCCGCCGAACAC19140.22653380786287725No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT19000.22487682076252177No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT18730.22168120278326492No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG18690.22120777789744905No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC18630.22049764056872534No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA18380.21753873503237633No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG17370.20558475666552653No Hit
ATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC16950.20061379536446022No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA16640.19694475249938753No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA16610.19658968383502565No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC16060.1900800916550579No Hit
CTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTC16010.18948831054778809No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC15990.18925159810488018No Hit
GTCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC15730.18617433634707725No Hit
GTGCAGCATGGGATGACAATTTGAGTGGTTGGGTGTTCGGCGGAGGGACT15410.18238693726055058No Hit
GAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCT15310.18120337504601097No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA15260.18061159393874118No Hit
GGCCATCAGTGGGCTCCGGTCCGAGGATGAGGCTGATTATTACTGTGCAG15050.17812611328820804No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCGGTCCGAGGATG14940.1768241948522145No Hit
GTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGC14340.16972282156497698No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGG14230.16842090312898345No Hit
CTCTTGTTCTGGAAGCAGATCCAACATCGGCAATAATTATGTAGACTGGT14170.16771076580025968No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA13810.16344994182791714No Hit
ATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTG13780.16309487316355528No Hit
GTGCCAGACTTGGAGCCAGAGAATCGGTCAGGGACCCCGGAGGACCGCTG13710.1622663796133776No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC13700.1621480233919236No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTG13420.15883404919121275No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC13350.15800555564103505No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC13330.15776884319812715No Hit
GTCCTCCGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAG13280.15717706209085733No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT13230.15658528098358754No Hit
ATTTAGTCCCTCCGCCGAACACCCAACCACTCAAATTGTCATCCCATGCTGCACAGTAATAATCAGCCTCATCCT13110.15516500632614003No Hit
GGGTCACCATCTCTTGTTCTGGAAGCAGATCCAACATCGGCAATAATTAT13060.15457322521887024No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT13000.1538630878901465No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC12850.1520877445683371No Hit
GTCCGAGGATGAGGCTGATTATTACTGTGCAGCATGGGATGACAATTTGAGTGGTTGGGTGTTCGGCGGAGGGA12840.15196938834688314No Hit
GTCAGGGACCCCGGAGGACCGCTGATTATTCCTATAGATGAGTAGTTTGG12810.1516143196825213No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCAATTTAGTC12590.14901048281053417No Hit
GGACTAAATTGACCGTCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAG12570.14877377036762626No Hit
GCATGGGATGACAATTTGAGTGGTTGGGTGTTCGGCGGAGGGACTAAATTGACCGTCCTACGTCAGCCCAAGG12540.14841870170326438No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT12480.14770856437454064No Hit
GTTTGGGGGCCGTTGGGGCCGCTCCTGGAAATTGCTGGTACCAGTCTACATAATTATTGCCGATGTTGGATCTG12450.14735349571017875No Hit
GTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTC12340.1460515772741852No Hit
CCTCCGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCGGT12120.14344774040219813No Hit
GGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGT12020.14226417818765852No Hit
CTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTG11890.14072554730875708No Hit
TAATTATGTAGACTGGTACCAGCAATTTCCAGGAGCGGCCCCAACGGCCC11780.13942362887276352No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG11540.1365830795578685No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC11310.13386088646442745No Hit
GCCCCAACGGCCCCCAAACTACTCATCTATAGGAATAATCAGCGGTCCTCCGGGGTCCCTGACCGATTCTCTGG11310.13386088646442745No Hit
GAGGGTCACCATCTCTTGTTCTGGAAGCAGATCCAACATCGGCAATAATT11290.13362417402151952No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG11280.13350581780006557No Hit
CTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAACGTCTGCGACCCCCGGACAGAGGGTCAC11260.13326910535715764No Hit
CCCCGGAGGACCGCTGATTATTCCTATAGATGAGTAGTTTGGGGGCCGTT11250.13315074913570368No Hit
GTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCT11220.13279568047134183No Hit
GGTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGG11000.13019184359935473No Hit
AGTCTGTGCTGACTCAGCCACCCTCAACGTCTGCGACCCCCGGACAGAGG10830.12817978783463743No Hit
GATTATTCCTATAGATGAGTAGTTTGGGGGCCGTTGGGGCCGCTCCTGGA10640.1259310196270122No Hit
GGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCT10620.1256943071841043No Hit
GATCCAACATCGGCAATAATTATGTAGACTGGTACCAGCAATTTCCAGGAGCGGCCCCAACGGCCCCCAAACTA10440.12356389519793302No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA10440.12356389519793302No Hit
CTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTC10410.12320882653357114No Hit
GTAATAGACAGCCGTGTCCTCACGTCTCGGGAGGTCCATTTGCAAAAATA10340.12238033298339343No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA10150.12013156477576822No Hit
GGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAAC10110.11965813988995239No Hit
TTACTGTGCAGCATGGGATGACAATTTGAGTGGTTGGGTGTTCGGCGGAG10100.11953978366849843No Hit
GCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC10050.11894800256122863No Hit
CGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC9900.11717265923941925No Hit
TCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAA9880.11693594679651133No Hit
GTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCA9860.11669923435360342No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9860.11669923435360342No Hit
GGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCAATTTAGTCCCTCCGCCGAACACC9770.11563402836051778No Hit
GGCCCAGTCTGTGCTGACTCAGCCACCCTCAACGTCTGCGACCCCCGGAC9630.11397704126016237No Hit
GATGAGTAGTTTGGGGGCCGTTGGGGCCGCTCCTGGAAATTGCTGGTACC9590.11350361637434653No Hit
CCCCCGGACAGAGGGTCACCATCTCTTGTTCTGGAAGCAGATCCAACATC9560.11314854770998464No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG9410.11137320438817527No Hit
CCGTTGGGGCCGCTCCTGGAAATTGCTGGTACCAGTCTACATAATTATTG9350.11066306705945152No Hit
TCAGTGGGCTCCGGTCCGAGGATGAGGCTGATTATTACTGTGCAGCATGG9310.11018964217363568No Hit
GGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTG9280.10983457350927381No Hit
GTCTGTGCTGACTCAGCCACCCTCAACGTCTGCGACCCCCGGACAGAGGG9170.10853265507328026No Hit
GGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCC9090.10758580530164857No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGC9040.10699402419437878No Hit
GGATGACAATTTGAGTGGTTGGGTGTTCGGCGGAGGGACTAAATTGACCGTCCTACGTCAGCCCAAGGCTGCCCC9030.10687566797292483No Hit
GACAGAGGGTCACCATCTCTTGTTCTGGAAGCAGATCCAACATCGGCAAT8990.10640224308710898No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG8980.10628388686565503No Hit
CCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGG8910.10545539331547732No Hit
ATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCC8890.10521868087256941No Hit
GGAATAATCAGCGGTCCTCCGGGGTCCCTGACCGATTCTCTGGCTCCAAG8890.10521868087256941No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG8850.10474525598675358No Hit
ACGTAGGACGGTCAATTTAGTCCCTCCGCCGAACACCCAACCACTCAAAT8850.10474525598675358No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC8790.10403511865802982No Hit
GCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACT8760.10368004999366794No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCT8730.10332498132930606No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC8680.10273320022203627No Hit
GGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGG8590.10166799422895063No Hit
GATGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG8570.10143128178604273No Hit
GTCTATTACTGTGCCAAAGATCTAGGAGGATACGCTTATGGTTTTGAAAGACTTGATTCTTACGGTCTGGACGT8560.10131292556458876No Hit
ACATAATTATTGCCGATGTTGGATCTGCTTCCAGAACAAGAGATGGTGAC8520.10083950067877293No Hit
CCTCAGCCTCCCTGGCCATCAGTGGGCTCCGGTCCGAGGATGAGGCTGAT8500.10060278823586502No Hit
CAATTTGAGTGGTTGGGTGTTCGGCGGAGGGACTAAATTGACCGTCCTACGTCAGCCCAAGGCTGCCCCCTCGGT8490.10048443201441107No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC2200.046.8676342
GGTATCA7050.046.385061
GTGGTAT2200.045.3752441
GTATCAA17200.040.626671
TATCAAC19350.036.056992
TCTGGAG5350.035.80179670
AATGGTA1151.0625237E-535.6906170
CGCGCGG400.00264229934.375724
TCAACGC20400.033.850134
ATCAACG20550.033.601063
AACGCAG20950.033.125456
CAACGCA21000.032.882985
TAGGATC652.2896764E-531.90907740
AGAGTAC22600.030.85718511
CGCAGAG22400.030.8259728
GACGGAC450.00471934230.5362838
CAGAGTA23150.029.97568110
ACGCAGA23200.029.912857
ATTGTCT3200.029.77701264
GCAGAGT23350.029.7189339
Produced by FastQC (version 0.11.5)