FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761478

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761478
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1311713
Sequences flagged as poor quality0
Sequence length20-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG26930.20530405660384551No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA47100.040.610771
GGTATCA19800.039.0894851
TATCAAC53150.035.9034042
TCAACGC56650.033.920624
CAACGCA56750.033.8608475
ACCGTTA750.003043585733.3976670
ATCAACG57550.033.2742843
AACGCAG57700.033.24436
ACGCAGA59250.032.2021067
TGGTATC7400.030.8527552
CGCAGAG62150.030.6446938
AGAGTAC62150.030.53854611
CAGAGTA63200.030.02774610
GCAGAGT65950.028.8789659
AGTACGG50900.027.91808713
GAGTACG51350.027.67343112
CGCTAAG807.411165E-526.86294763
TACGGGT8300.026.68921515
GTGGTAT8700.026.6494921
GTAACGG950.00966451626.36657370
Produced by FastQC (version 0.11.5)