FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761481

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761481
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences274284
Sequences flagged as poor quality0
Sequence length20-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGT2870.10463607064210818No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG451.7329803E-646.46032340
GGTATCA5450.042.2636641
GCCGTCT503.3579436E-642.20219846
ATGCCGT503.410778E-642.10653744
CGTATGC503.5231315E-641.90862741
TCTCGTA503.67153E-641.6580138
GATCTCG655.318161E-737.25311736
CGAGACA754.6575224E-836.17086423
CTCGTAT601.2649776E-534.7865239
ACGAGAC601.5141637E-533.88544522
TATGCCG652.08064E-532.3468743
TATTCTA1152.2757615E-531.91250270
TATACAC751.9217896E-631.6091143
CGTCAGA652.6275215E-531.2617655
GTATCAA11250.031.0126911
CTAGTGC450.005054321630.103923
CCACGAG704.3414046E-529.04466420
CTTATAC704.3522236E-529.0340731
CAGCGTC704.3576416E-529.028782
TCCGAGC704.3576416E-529.0287813
Produced by FastQC (version 0.11.5)