FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761483

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761483
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1449791
Sequences flagged as poor quality0
Sequence length20-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15550.040.384831
GTATCAA36350.038.7058871
ATCAACG43150.032.6017383
TATCAAC43600.032.5845262
TCAACGC43200.032.560634
CAACGCA43350.032.4479685
AACGCAG45500.031.0644426
TCGTATG1501.8189894E-1230.0139540
CGTATGC1650.029.4088641
ACGCAGA48550.029.1129157
CGCAGAG49400.028.6814338
AGAGTAC49650.028.46791811
TATACCG500.00794935227.446235
CAGAGTA51650.027.43210
GCAGAGT52050.027.2870989
CTCGTAT1759.094947E-1225.70208239
AGTACGG38700.025.353213
GAGTACG39000.024.98224412
TAACGGC2650.024.74053836
TTAACGG2650.024.71567335
Produced by FastQC (version 0.11.5)