FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761486

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761486
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1816607
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAGCAGTATGGTGGCTCATTTTACACTTTTGGCCAGGGGA58490.3219738776741475No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT53450.2942298471821368No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA44960.24749436724619028No Hit
GTAATATACGGCCGTGTCTGCGGCGGTCACAGAGGTCAGCTTCAGGGAGA43610.24006293050725888No Hit
CTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA32780.18044629355716452No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC32770.1803912458776169No Hit
GTCTCTGGTGGCTCCATCAGTAGTTACTACTGGAGCTGGATCCGGCAGCC32390.17829943405480655No Hit
GTGTATTACTGTCAGCAGTATGGTGGCTCATTTTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA32150.1769782897456632No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT32080.17659295598882974No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGTT31830.17521676400013872No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG31680.17439104880692413No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATT31250.17202399858637557No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT30100.16569351543839697No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC29100.16018874748363296No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC28070.15451883649022602No Hit
GTATATTACTGTGCGAGAACCGGTTTTTGGACTGGTTATTTTGACTTCTGGGGCCAGGGAACCCAGGTCACCGT27990.1540784550538449No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA27900.15358302593791615No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG26100.1436744436193409No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG26040.14334415754205507No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCGGGGCCAGTCAGAGTCTTACCGGTCCGAGTCTTAGCGG25850.14229825163064988No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT25640.14114225036014943No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT25230.1388852954986962No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTTCTCCTGGTGGCAGCTCCCAGATGGGTC24060.1324447169916223No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC24040.13233462163252702No Hit
CTCCTGCGGGGCCAGTCAGAGTCTTACCGGTCCGAGTCTTAGCGGCGCCTACTTAGCCTGGTACCAGCAGAAAC23880.13145385875976476No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT23000.12660966295957243No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCT22930.12622432920273896No Hit
GAGTCTTACCGGTCCGAGTCTTAGCGGCGCCTACTTAGCCTGGTACCAGCAGAAACCTGGCCTGGCGCCCAGGCT22920.1261692815231913No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT21870.12038927517068909No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT20570.11323307682949586No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC20560.11317802914994823No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC20520.11295783843175766No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG20510.11290279075221003No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG20430.1124624093158289No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG20370.11213212323854306No Hit
GACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATTTTGCAGTGTA20360.11207707555899542No Hit
TACTTAGCCTGGTACCAGCAGAAACCTGGCCTGGCGCCCAGGCTCCTCATCTATGATGCATCCAACAGGGCCA20220.11130640804532846No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC20120.11075593124985207No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT20070.11048069285211386No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG20060.11042564517256621No Hit
GTCAGAGTCTTACCGGTCCGAGTCTTAGCGGCGCCTACTTAGCCTGGTAC19830.1091595485429705No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG19820.10910450086342284No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCA19600.10789345191337477No Hit
GCTCACTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT19060.1049208772178022No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA19040.10481078185870692No Hit
CACCTGCACTGTCTCTGGTGGCTCCATCAGTAGTTACTACTGGAGCTGGATCCGGCAGCCCCCAGGGAAGGGC19010.10464563882006399No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA19000.10459059114051636No Hit
GTATGGTGGCTCATTTTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT18960.10437040042232579No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG18370.10112258732901502No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA35200.043.237321
GGTATCA12900.041.013681
TATCAAC40500.037.4643332
ATCAACG41800.035.9617653
TCAACGC42250.035.57584
CAACGCA42750.035.2428935
AACGCAG43700.034.5552756
AGAGTAC45200.033.4835411
CGCAGAG45550.033.3006828
AACTGTG27600.032.87644270
GCAGAGT49150.031.0012139
ACGCAGA49700.030.4526677
CAGAGTA52850.028.76590210
AGTACGG47100.028.70981613
GAGTACG47500.028.46883412
TGGATTG16800.028.19707770
TACGGGG24550.025.86634815
GTACGGG54550.024.72594514
TACGGGA22000.024.65183815
ACGGGGC4350.024.46375816
Produced by FastQC (version 0.11.5)