FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761487

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761487
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1031626
Sequences flagged as poor quality0
Sequence length20-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA30100.039.5568351
CTCGTAT1700.038.6708339
CGTATGC1750.037.66567241
TCTCGTA1800.036.483138
TATCAAC35000.034.012282
TCAACGC35350.033.675534
CAACGCA35350.033.578765
GGTATCA13250.033.5688361
CGAGACG1950.033.34679823
ATCAACG36200.032.8848043
AACGCAG36350.032.745936
TCGTATG2050.032.1078540
ACGCAGA38250.031.029917
AGAGTAC38750.030.54125611
ACGGACT2150.030.28442627
CGCAGAG39650.029.9342778
TATCTCG2200.029.79144336
GACGGAC2250.028.92861626
CAGAGTA41950.028.53767410
GAGTACG30700.028.07660912
Produced by FastQC (version 0.11.5)