FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761491

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761491
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1027666
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTGGGGTGTCTCCACCATGGCCTGGACCCCTCTCTGGCTCACTCTCC14930.14528066511882265No Hit
GACTTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCACAACTTTACCA14250.13866372926612341No Hit
CTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGT13910.13535526133977382No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT12420.12085638719194758No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT10720.10431404756019952No Hit
GCCTTGGGCTGACTTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCAC10580.10295173723758498No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG3700.045.76218470
GTCGTAT405.2323965E-542.79652439
GGTATCA11250.039.3875921
TCGTATG2450.039.2314740
GTATCAA24250.037.3757631
CTTATAC2900.035.884071
CGTATGC2850.035.01866541
TATACAC3100.034.6450963
TATCAAC26350.034.008242
GTGGTAT3600.032.63651
TTATACA3200.032.513612
ACGAGAC3350.032.07056422
TCAACGC28950.031.1827854
ATCAACG29150.030.9718553
CAACGCA29800.030.405965
GCCGTCT2900.030.06710646
AACGCAG30300.029.9012996
CACGAGA3650.029.4303321
TGGTATC4250.029.2190632
ATCTCGT2800.029.19545637
Produced by FastQC (version 0.11.5)