FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761492

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761492
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1027666
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG34370.33444718420187103No Hit
GTTCTGGGGTGTCTCCACCATGGCCTGGACCCCTCTCTGGCTCACTCTCC15380.14965951972722655No Hit
GACTTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCACAACTTTACCA11940.11618560894298341No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT10880.10587097364318757No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT10790.1049952027215068No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG10280.10003250083198238No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11200.039.857521
GTATCAA26450.038.322861
TATCAAC29200.034.4468652
ATCAACG30600.032.858073
TCAACGC30500.032.750814
CAACGCA31550.031.8764365
AACGCAG32200.031.3370786
GTGGTAT4350.030.0920661
AGAGTAC33950.029.9207111
CGCAGAG34300.029.6139558
CAGAGTA34300.029.41848410
GTACGCA606.3679484E-427.970191
TGGTATC4650.027.3994582
ACGCAGA36750.027.3660327
AGTACGG33100.027.34669313
GAGTACG33750.026.82001312
CTCGTAT859.384916E-525.94544260
CACTCGT1001.03295315E-525.47635758
TACGGGA12450.023.96684315
GTACGGG37950.023.85179114
Produced by FastQC (version 0.11.5)