FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761493

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761493
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences543202
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTACCG200.00803900950.60230322
TCGTATG704.110916E-1044.69111640
CTCGTAT803.274181E-1143.35502239
ACGAGAC1150.038.13506722
GTATCAA27450.033.7714231
GGTATCA13900.033.46751
CGTATGC957.95626E-933.01492341
GTGGTAT5250.031.4626831
AATCGTA850.005014617530.15293970
TATACAC1251.6370905E-1029.6648163
ATGCCGT1202.2719178E-929.19653144
TATCAAC33750.028.9656452
TCAACGC34500.028.4336684
ATCAACG34750.028.2291093
GCCGTCT1253.3651304E-928.14177746
CGAATCG550.007060383428.11746468
CAACGCA34900.028.107785
GAATTAG606.2047865E-428.0916821
AACGCAG35300.027.9751196
CCGTCTT1403.6379788E-1027.6977947
Produced by FastQC (version 0.11.5)