FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761494

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761494
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences543202
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9480.17452071236851116No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA25800.039.6653021
GGTATCA10750.035.304741
TATCAAC30350.033.3469162
TCAACGC30100.033.043354
AACGCAG30600.032.945656
ATCAACG30750.032.45193
CAACGCA30800.032.4022035
ACGCAGA32250.031.260067
AGAGTAC32000.030.98138611
ATGCGTA500.00449643330.83709368
CCGTATG450.00472522830.5264834
CAGAGTA33050.030.19630210
CGCAGAG33700.029.4131098
GCAGAGT33950.029.196529
GTAGTTT2053.292189E-828.52280870
GAGTACG29200.027.34968412
AGTACGG28700.027.23662613
CCCGAAA708.766716E-426.47767667
ACGGGGC3350.025.26029616
GTACGGG31750.024.5136114
Produced by FastQC (version 0.11.5)