FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761495

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761495
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1059438
Sequences flagged as poor quality0
Sequence length20-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA28350.040.4974251
GGTATCA11600.039.589721
CTCGTAT1400.039.5145939
TATCAAC32450.035.27172
GTGGTAT3900.034.27171
GCGACCG400.002684017934.2668511
TCAACGC33700.034.065094
CAACGCA33800.033.8629235
TCGTATG1850.033.67126540
ATCAACG34300.033.5675283
AACGCAG34900.032.795616
CGTATGC1950.031.98264541
TCTCGTA1850.031.74767538
ACGCAGA37250.030.7266247
AGAGTAC38200.029.96106711
CGCAGAG38250.029.8337238
CGAGACG2300.029.81273723
CAGAGTA38450.029.67854310
AGGGGGG4200.029.32701770
GCAGAGT40750.028.0875269
Produced by FastQC (version 0.11.5)