FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761498

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761498
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences870808
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20320.23334650118051278No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACGT200.007531359351.4468331
GGTATCA9100.037.5989271
GTATCAA20600.036.5403181
TATCAAC24850.030.529462
CAACGCA24400.030.5252655
TCAACGC24450.030.462844
GTGGTAT3750.030.1092221
ATCAACG24900.029.910593
AACGCAG25600.029.3613136
TGGTATC4050.028.689072
ACGCAGA26500.028.106287
CGCAGAG27600.026.8623148
AGAGTAC28600.026.16349811
CAGAGTA29900.025.02307910
TCGGTTT2051.3886074E-624.55461170
GCAGAGT30350.024.5409059
TATAGAC851.5419842E-424.1157153
AGTACGG27050.023.49569713
GAGTACG27550.023.31733312
TAACGGC1052.319683E-522.94862736
Produced by FastQC (version 0.11.5)